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Comments on Jurgen Hench et al. (2008) European Worm Meeting "Detection of Embryonic Gene Expression Dynamics" (0)
Overview
Jurgen Hench, Johan Henriksson, Martin Luppert, Konstantin Cesnulevicius, Krai Meemon, David Baillie, & Thomas Burglin (2008). Detection of Embryonic Gene Expression Dynamics presented in European Worm Meeting. Unpublished information; cite only with author permission.
Gene expression is extensively studied in C. elegans at different levels.. While protein and RNA detection methods reveal localization and changes in. expression on a rather rough temporal scale, fluorescent reporter. transgenes allow expression studies in vivo. Even though those reporters. are unable to represent the final protein expression they give detailed. information suitable to construct models of regulatory networks.. In addition to the recently published genome wide promoterome screens (Hunt-. Newbury et al., 2007; Dupuy et al., 2007; Reece-Hoyes et al., 2007) we. propose a system to automatically obtain, map and compare embryonic. promoterome data. In addition to the localization we emphasize on the. detection and quantification of rapid changes in transcription during cell. differentiation. We have focused on genes known or expected to be expressed. during embryogenesis such as homeodomain containing transcription factors.. We find that several homeobox genes show highly dynamic expression patterns. that are easily overlooked using established methods.. We have screened over 80 genes in over 200 individual transgenic animals.. The expression patterns are normalized to a standard spatiotemporal (4D). coordinate system and can be compared. The temporal expression patterns. obtained from different transgenic reporter individuals for a particular. promoter usually correlate with r > 0.98. The success rate with our system. to obtain a complete 4D expression pattern from a single transgenic embryo. is greater than 95%.