Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Makoto Horikawa et al. (2008) C.elegans Aging, Stress, Pathogenesis, and Heterochrony Meeting "Daf-16 Regulates Fat Storage of Nematode Bidirectionally under the Control of PUFA"

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Makoto Horikawa et al. (2008) C.elegans Aging, Stress, Pathogenesis, and Heterochrony Meeting "Daf-16 Regulates Fat Storage of Nematode Bidirectionally under the Control of PUFA" (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00032634

    Makoto Horikawa, & Kazuichi Sakamoto (2008). Daf-16 Regulates Fat Storage of Nematode Bidirectionally under the Control of PUFA presented in C.elegans Aging, Stress, Pathogenesis, and Heterochrony Meeting. Unpublished information; cite only with author permission.

    DAF-16, a FoxO homolog of nematode, induces an expression of fatty acid metabolic genes fat-7 and elo-2, resulting in the accumulation of fat storage. However, functional interaction among daf-16, fatty acid metabolism and fat storage remains to be clarified. Recently, we clarified that introduction of fat-2-RNAi and fat-6-RNAi decreased fat accumulation of nematode. RNAi of fat-2, fat-6 and elo-2 raised transcriptional activity of daf-16, particularly, RNAi effect of fat-2 which was oleic acid desaturase was remarkable. This result contradicts a past report that daf-16 was associated with an increase of fat storage. Therefore we examined functional interaction of daf-16 and fat storage. Induction of dauer formation at 25 degrees centigrade of daf-2 mutant (e1370) resulted in the increase of fat storage in L2 larva, and the decrease of fat storage in L4 larva. In daf-16 deficit mutant (mgDF50), fat storage level which reduced by RNAi of fat-2, fat-7 and elo-2, was restored to an equal level to N2-RNAi (-). Furthermore, transcriptional activity of daf-16 was suppressed by addition of linoleic acid or alpha-linolenic acids to fat-2 RNAi worm, and fat storage recovered at the same time. These results suggest that daf-16 acted to an increase of fat storage in Dauer larva, and a suppression of fat storage in adult worm. In addition, functional interaction between daf-16 and fatty acid metabolism may undergo some controls by possibly PUFA (Poly-unsaturated fatty acids).


    Tip: Seeing your name marked red? Please help us identify you.