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Comments on Yamei Wang (2008) "Transcriptional regulation of life span regulators DAF-16 and SIR-2.1 in Caenorhabditis elegans" (0)
Overview
Yamei Wang (2008). Transcriptional regulation of life span regulators DAF-16 and SIR-2.1 in Caenorhabditis elegans presented in a meeting. Unpublished information; cite only with author permission.
"Aging is a complex and fascinating process. Many single gene mutations, particularly genes along the daf-2/insulin-like signaling pathway, have been shown to be able to change the life span in diverse organisms. DAF-16, a forkhead transcrioption factor in Caenohabiditis elegans is the major output of the daf-2/insulin-like signaling pathway and a key mediator of longevity. Another important regulator of longevity, the conserved SIR2 protein regulates life span in both yeast and worms. In both organisms overexpression of SIR2 can extend life span and in C. elegans this life span extension is dependent on DAF-16. Previously we have shown that DAF-16 and SIR-2.1 have overlapping and distinct expression pattern in consistent with their unique and redundant functions in life span regulation. To better understand how the spatial and temporal expression pattern of daf-16 and sir-2.1 genes is differentially regulated through a network of transcription factors, we undertook a yeast one-hybrid screen by using a AD-cDNA library and a AD-TF mini library. Currently, we are double checking the positive yeast colonies. Soon we will identify the double positive yeast colonies by yeast colony PCR. Once the identity of positive colonies is revealed, we will analyze the effect of RNAi of those positive regulators on the gene expression of daf-16 and sir-2.1. By using this approach, we are hoping to identify the transcription factors are expressed in overlapping domains to regulate daf-16 and sir-2.1 gene expression, which will shed light on how these two key longevity regulators function coordinatively in C. elegans."
