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Resources » Paper

Li J et al. (2007) J Hum Genet "Identification and characterization of human uracil phosphoribosyltransferase (UPRTase)."

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  • Comments on Li J et al. (2007) J Hum Genet "Identification and characterization of human uracil phosphoribosyltransferase (UPRTase)." (0)

  • Overview

    PMID:
    17384901
    DOI:
    10.1007/s10038-007-0129-2
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00029217

    Li J, Huang S, Chen J, Yang Z, Fei X, Zheng M, Ji C, Xie Y, & Mao Y (2007). Identification and characterization of human uracil phosphoribosyltransferase (UPRTase). J Hum Genet, 52, 415-22. doi:10.1007/s10038-007-0129-2

    Uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate, is important in the pyrimidine salvage pathway and is an attractive target for rational drug design by incorporation of prodrugs that are lethal to many parasitic organisms specifically. So far, uracil phosphoribosyltransferase has been reported in Arabidopsis thaliana only, not in mammals. In this study, a novel uracil phosphoribosyltransferase family cDNA encoding a 309 amino acid protein with a putative uracil phosphoribosyltransferase domain was isolated from the human fetal brain library. It was named human UPRTase (uracil phosphoribosyltransferase). The ORF of human UPRTase gene was cloned into pQE30 and expressed in Escherichia coli M15. The protein was purified by Ni-NTA affinity chromatography, but UPRTase activity could not be detected by spectrophotometry. RT-PCR analysis showed that human UPRTase was strongly expressed in blood leukocytes, liver, spleen, and thymus, with lower levels of expression in the prostate, heart, brain, lung, and skeletal muscle. Subcellular location of UPRTase-EGFP fusion protein revealed that human UPRTase was distributed in the nucleus and cytoplasm of AD293 cells. Evolutional tree analyses of UPRTases or UPRTase-domain-containing proteins showed that UPRTase was conserved in organisms. UPRTases of archaebacteria or eubacterium had UPRTase activity whereas those higher than Caenorhabditis elegans, which lacked two amino acids in the uracil-binding region, had no UPRTase activity. This means that human UPRTase may have enzymatic activity with another, unknown, factor or have other activity in pyrimidine metabolism.

    Authors: Li J, Huang S, Chen J, Yang Z, Fei X, Zheng M, Ji C, Xie Y, Mao Y


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