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Resources » Paper

Belogrudov GI et al. (2001) Arch Biochem Biophys "Yeast COQ4 encodes a mitochondrial protein required for coenzyme Q synthesis."

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  • Comments on Belogrudov GI et al. (2001) Arch Biochem Biophys "Yeast COQ4 encodes a mitochondrial protein required for coenzyme Q synthesis." (0)

  • Overview

    PMID:
    11469793
    DOI:
    10.1006/abbi.2001.2448
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00028293

    Belogrudov GI, Lee PT, Jonassen T, Hsu AY, Gin P, & Clarke CF (2001). Yeast COQ4 encodes a mitochondrial protein required for coenzyme Q synthesis. Arch Biochem Biophys, 392, 48-58. doi:10.1006/abbi.2001.2448

    The COQ4 gene coding for a component of the coenzyme Q biosynthetic pathway in the yeast Saccharomyces cerevisiae was cloned by a functional complementation of a Q-deficient mutant strain. Yeast coq4 mutant strains harboring the COQ4 gene on either single- or multicopy plasmids acquired the ability to grow on media containing a nonfermentable carbon source, synthesize Q(6), and respire. COQ4 encodes a polypeptide containing 335 amino acids with a calculated molecular mass of 38.6 kDa. By Western blot analysis with a specific antiserum, Coq4p was shown to peripherally associate with the matrix face of the mitochondrial inner membrane. The putative mitochondrial-targeting sequence present at the amino-terminus of the polypeptide efficiently imported it to mitochondria in a membrane-potential-dependent manner. Steady-state levels of COQ4 mRNA were increased during growth on glycerol-containing medium, in accordance with a function in Q biosynthesis. The function of Coq4p is unknown, although its presence is required to maintain a steady-state level of Coq7p, another component of the Q biosynthetic pathway. The results presented here, along with those available from literature, are discussed in light of the recently proposed existence of a multisubunit complex functioning in Q biosynthesis (A. Y. Hsu, T. Q. Do, P. T. Lee, and C. F. Clarke, 2000, Biochim. Biophys. Acta 1484, 287-297).


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