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Resources » Paper

Vida Praitis et al. (2006) Development & Evolution Meeting "Characterizing a temperature-sensitive mutation required for tubule morphogenesis in C. elegans"

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00028064

    Vida Praitis, Michael Miller, Juliette Mushi, Zelealem Yilma, Lensa Yohannes, Angela Schacht, & Sarah Kniss (2006). Characterizing a temperature-sensitive mutation required for tubule morphogenesis in C. elegans presented in Development & Evolution Meeting. Unpublished information; cite only with author permission.

    During C. elegans morphogenesis, epithelial cells derived from the hypodermis, buccal cavity, and pharynx must attach to each other to form a continuous tube. While much of this process is understood at the cellular level, the molecules required to bring these epithelial sheets together are still being characterized. We are characterizing ru5, a temperature-sensitive embryonic lethal mutation that prevents buccal and pharyngeal epithelial cells from forming stable connections during morphogenesis, resulting in embryos with the Pun (pharynx unattached) phenotype. In ru5 mutants, the invagination that marks formation of the buccal cavity does not occur in ru5 embryos. Temperature shift experiments show the altered gene product is required midway through embryogenesis, when buccal and pharyngeal cells come together to form the mouth. To further characterize the ru5 phenotype, we examined the expression pattern of proteins found in the pharynx and head hypodermis during this stage of development. Our results indicate that developmental events, such as the establishment of the pharyngeal primordium, hypodermal enclosure, and reorientation of the anterior pharynx, appear normal. However, the localization of AJM-1 and SMA-1, two proteins that localize to apical domains in epithelial cells (Praitis, Ciccone, & Austin, 2005; Koppen, et al, 2001), is abnormal in the arcade cells and in the head hypodermis. These results suggest the failure to form a stable epithelium is due to defects in the organization, movement, or shape of the arcade cells or anterior hypodermis. We have mapped the ru5 locus and are currently working to identify the altered gene.


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