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Resources » Paper

Christopher Richie et al. (2006) Development & Evolution Meeting "Identification and characterization of separase cofactors in C. elegans"

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00027967

    Christopher Richie, & Andy Golden (2006). Identification and characterization of separase cofactors in C. elegans presented in Development & Evolution Meeting. Unpublished information; cite only with author permission.

    Accurate chromosome segregation requires separation of the sister chromatids during the metaphase-to-anaphase transition. This is achieved when the anaphase-promoting complex (APC) brings about the destruction of securin, an inhibitor of the cysteine protease known as separase. Once activated, separase can then cleave the SCC-1 protein, a member of the cohesin complex that holds the sister chromatids together. In C. elegans, the sep-1(e2406) temperature-sensitive mutant allele of separase causes a pleiotropic phenotype when worms are grown at 20°C (Siomos et al, 2001). In addition to complete sterility and embryonic lethality, sep-1(e2406) also causes developmental abnormalities in the germ line, somatic gonad, and the vulva. We have recently isolated new temperature sensitive alleles of sep-1 (ax110 and ax521) and are currently characterizing their respective phenotypes. In an effort to identify novel regulators and substrates of separase, we have performed a suppressor screen to select for mutations that can rescue the sterility and embryonic lethality associated with the sep-1(e2406) allele when grown at the restrictive temperature. One such allele, designated supE, suppresses sep-1(e2406 and ax110), but acts as an enhancer of ax521. We have mapped supE to a relatively small physical interval with 53 predicted coding sequences, and are currently attempting to identify candidates by RNAi. To date, RNAi of one candidate in the region appears to suppress both sep-1(e2406 and ax110) quite well. This data will be presented at the meeting.


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