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Resources » Paper

Marketa Kostrouchova et al. (2006) European Worm Meeting "Valproic Acid Affects Gene Expression and Development in Caenorhabditis elegans"

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00027458

    Marketa Kostrouchova, Petr Liby, Marta Kostrouchova, & Zdenek Kostrouch (2006). Valproic Acid Affects Gene Expression and Development in Caenorhabditis elegans presented in European Worm Meeting. Unpublished information; cite only with author permission.

    Marketa Kostrouchova1, Petr Liby1, Marta Kostrouchova2 and Zdenek Kostrouch1. Valproic acid (VPA, 2-propylpentanoic acid), a drug widely used in the treatment of epilepsy and bipolar disorder, has been shown to posses anti-cancer activity. VPA suppresses tumor growth, induces tumor cell differentiation and reduces the formation of metastases. Multiple mechanisms were shown to be part of VPAs mode of action, including inhibition of histone deacetylase activity, inhibition of glycogen synthase 3 and interaction with the regulation of nuclear hormone receptors. We studied the effect of VPA on the development and gene expression in C. elegans. VPA delivered to worms by microinjections to the ovarial syncytium or by incubating worms in various concentrations of VPA (1 to 10 mM) caused a dose dependent delay of larval development and lethality in two developmental stages: in late embryogenesis and in L1 larval stage. Surprisingly, most animals were able to overcome the effect of VPA, suggesting a compensatory or balancing mechanism. Microarray experiments show that genes affected by VPA can be divided to at least three different categories suggesting multiple mechanisms to be involved in VPA mode of action. Acknowledgement: We thank Dr. M.W. Krause for support and advice. We thank the NIDDK Microarrays facility for performing the microarrays analysis. The work was supported by the grant 303/03/0333 from the Czech Science Foundation, by the grant NC-7554 from the Ministry of Health of the Czech Republic and by the grant 0021620806 from the Ministry of Education, Youth and Sports of the Czech Republic.


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