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Resources » Paper

Tsuyoshi Kawano et al. (2005) International Worm Meeting "Gene-disruptions and physiological functions of C. elegans insulin-like genes Ceinsulin-1 and -2"

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  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00025466

    Tsuyoshi Kawano, Ikuko Tanaka, Naoya Kataoka, Yasuo Kimura, Masaji Ishiguro, Keiko Gengyo-Ando, & Shohei MItani (2005). Gene-disruptions and physiological functions of C. elegans insulin-like genes Ceinsulin-1 and -2 presented in International Worm Meeting. Unpublished information; cite only with author permission.

    In C. elegans, the insulin-signaling regulates the diapause, longevity and fat metabolism via the DAF-2, a homolog of insulin/IGF-I receptor. Our search for the DAF-2 ligands revealed two insulin/IGF-like peptides named Ceinsulin-1 (INS-18) and -2 (INS-17). First, in order to obtain structural information of the peptides, we performed a computer modeling using the Discover III/Insight II. The predicted tertiary structures of the peptides were quite similar to each other, and their receptor-recognition surfaces matched significantly. Therefore, these peptides should be recognized by the same receptor. Next, to elicit the physiological function of the ins-17 and -18 genes, we disrupted the genes using the TMP/UV method. The following sib-screening yielded an ins-17 deletion mutant named tm790 and an ins-18 deletion mutant named tm339. The tm790 has deletion of approximately 0.6 kb on two exons in the F56.F3 gene and the tm339 possesses deletion about 1.4 kb corresponding to two of three exons in the T28B8.2 gene. Then, we measured the life span and dauer larva formation of the mutant animals. The tm339 exhibited a slightly extended life span, while the tm790 showed a little bit of short life span. Both of the mutant animals formed no dauer larva under a normal growth-condition. However, in the presence of roughly purified diapause-inducing pheromone, the tm339 and tm790 formed more and less dauer larva, respectively, than the wild-type animal. Gengyo-Ando, K. and Mitani, S. (2000) BBRC 269: 64-69. Kawano, T., et al. (2000) BBRC 273: 431-436. Kawano, T., el al. (2003) BBB 67: 2678-2682.

    Affiliations:
    - Suntory Institute for Bio-organic Research
    - Dept. of Agriculture, Tottori Univ., Tottori, Japan
    - Dept. of Physiology, Tokyo Women's Medical Univ., School of Medicine


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