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Resources » Paper

P Yang et al. (2004) European Worm Meeting "IDENTIFICATION OF NOVEL GENES REQUIRED FOR Q NEUROBLASTS MIGRATION"

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  • Comments on P Yang et al. (2004) European Worm Meeting "IDENTIFICATION OF NOVEL GENES REQUIRED FOR Q NEUROBLASTS MIGRATION" (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00024740

    P Yang, & H Korswagen (2004). IDENTIFICATION OF NOVEL GENES REQUIRED FOR Q NEUROBLASTS MIGRATION presented in European Worm Meeting. Unpublished information; cite only with author permission.

    The neuroblasts QL and QR are born at similar anteroposterior positions on the left and right side of the animal and generate an identical set of descendants. Despite this similarity in lineage, the migration of the Q neuroblasts and their daughter cells (Q.d) is strikingly different between the two sides. Thus, QL and its descendants migrate towards the posterior, whereas QR and its daughter cells migrate towards the anterior. This difference in migration is specified by the left/right asymmetric expression of the homeobox gene mab-5. Thus, mab-5 is only expressed in the QL lineage, where it directs migration towards the posterior; mab-5 is not expressed in QR and its descendants, which results in migration in the default anterior direction. The expression of mab-5 in the QL lineage is in turn regulated by a canonical Wnt/?-catenin signaling pathway. We use the left/right asymmetric migration of the Q neuroblasts as a model to study the mechanism and regulation of the canonical Wnt pathway. In addition, we are interested in the cell migration and guidance mechanisms that direct the Q neuroblasts and their descendants towards their final positions. To identify novel genes that are required for Q neuroblast migration, we performed a large scale genetic screen. From a clonal screen of 12.900 haploid genomes, we obtained a total of 75 mutations that show defects in Q neuroblast migration. 48 of these show anterior localization of the QL.d, 12 show posterior localization of the QR.d and 14 show random localization of the QL and QR daughter cells. Using genetic mapping and complementation tests, we found that 35 of these mutations were allelic to know genes. Thus, we isolated 12 new alleles of egl-20/Wnt, 6 alleles of mab-5, 5 alleles of mig-21, 5 alleles of bar-1/?-catenin, 4 alleles of unc-40/DCC and single alleles of mig-13, ceh-20 and sqt-3. We are now focusing on 4 mutations that are probably alleles of novel genes that are required for correct Q neuroblasts migration.

    Affiliation:
    - Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht, The Netherlands


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