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Resources » Paper

H Pamjav et al. (1999) International C. elegans Meeting "Phylogenetic analysis of entomopathogenic nematode / bacterium symbiotic complexes by using PhastSystem PAGE PCR-RFLP of rDNA spacer sequences"

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  • Comments on H Pamjav et al. (1999) International C. elegans Meeting "Phylogenetic analysis of entomopathogenic nematode / bacterium symbiotic complexes by using PhastSystem PAGE PCR-RFLP of rDNA spacer sequences" (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00023514

    H Pamjav, D Triga, E Szallas, A Fodor, & Z Buzas (1999). Phylogenetic analysis of entomopathogenic nematode / bacterium symbiotic complexes by using PhastSystem PAGE PCR-RFLP of rDNA spacer sequences presented in International C. elegans Meeting. Unpublished information; cite only with author permission.

    Entomopathogenic nematode (EPN)species belonging to the Steinernema and Heterorhabditis genera are closely related to C. elegans (Blaxter et al., 1998). They form an intimate symbiotic complex with bacteria Xenorhabdus spp. and Photorhabdus spp., respectively, which are closely related to E. coli . The symbiosis is developmentally regulated: there is a direct cell-cell contact between the gut cell of the (infective) dauerlarva and the primary phase variant of the symbiotic bacteria. The symbiosis is taxon specific. Individual Xenorhabdus species used as symbionts by each Steinernema species and individual strains of Photorhabdus luminescens used as symbionts by each Heterorhabditis strains belonging to different species. The phylogenetic relations between species and strains have been analysed by RFLP of the internally transcribed spacer region of the respective rRNA operon. The PCR-amplified DNAs were digested by different restriction enzymes and the restriction patterns obtained by PhastSystem PAGE were compared. On the basis of the comparative analysis of the ITS1 - ITS2 patterns nematodes could be identified at species level. Xenorhabdus species could also be identified on the basis of the comparative analysis of the spacer region of the 16S - 23S rRNA operon. P. luminescens strains, belonging to different subclusters (Szallas et al., 1997) could be separated on the basis of the RFLP pattern of the spacer region of the 16S - 23S rRNA operon. Adams, B.J. (1998). Species conception and the evolutionary paradigm in modern nematology. J. nematol. 30, 1-21. Adams, B.J., Burnell, A.M. @ Powers,T.O. (1998). A phylogenetic analysis of Heterorhabditis based on ITS 1 DNA sequence data. J. nematol. 30, 22-39. Blaxter,R. et al.(1998).Molecular evolutionary framework for Phylum nematoda. Nature 392,71-75. Szallas E., Koch, H., Fodor,A.,Szentirmai, A., Nealson,K.H. Stackebrandt, E.(1997).Phylogenetic evidence for taxonomic heterogeneity of P.luminescens. IJSB 47, 402-407.


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