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Resources » Paper

Onami S et al. (1997) International C. elegans Meeting "CHANGES IN POLY(A) TAIL LENGTH OF MATERNAL MESSAGES."

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00022608

    Onami S, & Kohara Y (1997). CHANGES IN POLY(A) TAIL LENGTH OF MATERNAL MESSAGES presented in International C. elegans Meeting. Unpublished information; cite only with author permission.

    In Drosophila and Xenopus, lots of maternal mRNA have been reported whose translation is regulated through cytoplasmic polyadenylation; their poly(A) tails elongate when their translation is activated. Aiming to fish out the maternal mRNA of C. elegans whose translation is regulated through cytoplasmic polyadenylation, we have developed a method to assess the poly(A) tail length of given mRNA in embryos at a specific stage. First, we examined the poly(A) tail lengths of several known maternal mRNA whose translation is considered to be regulated. Maternal glp-1 mRNA exists in embryo before the first division and is detected in both blastomeres at 2cell stage, while GLP-1 protein first appears at 2cell stage and is restricted in AB blastomere(Evans et al., Cell 77:183). In order to examine the poly(A) tail length of glp-1 mRNA, total RNA was extracted from 15 embryos at the same stage. An RNA-oligonucleotide-tag was added to its 3'-end. This RNA was examined by RT-PCR using a glp-1-specific and a tag-specific primer. Results showed that glp-1 mRNA has poly(A) tail of -40 bases in matured oocyte while 2cell stage embryo contains glp-1 mRNA with longer poly(A) tail(>110bases). 2cell stage embryo also contains glp-1 mRNA with shorter poly(A) tail(-25bases). These results suggest that cytoplasmic polyadenylation is involved in the translational regulation of glp-1 mRNA. Fem-3 mRNA and skn-1 mRNA were examined and elongation of their poly(A) tails during embryogenesis was also observed. We are currently examining the exact time at which the poly(A) tails start to elongate. Also, we are examining their poly(A) tail lengths in AB and P1 blastomere.

    Affiliation:
    - Department of Genetics, The Graduate University for Advanced Studies, Mishima 411 Department of Genetics, The Graduate University for Advanced Studies, CREST and Gene Network Lab, National Institute of Genetics, Mishima 411


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