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Resources » Paper

Koeppen M et al. (1997) International C. elegans Meeting "ANALYSIS OF THE GENE ENCODING THE ANTIGEN RECOGNIZED BY THE MH27 ANTIBODY"

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  • Comments on Koeppen M et al. (1997) International C. elegans Meeting "ANALYSIS OF THE GENE ENCODING THE ANTIGEN RECOGNIZED BY THE MH27 ANTIBODY" (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00022451

    Koeppen M, Radice AD, Simske JS, Moran AN, & Hardin JD (1997). ANALYSIS OF THE GENE ENCODING THE ANTIGEN RECOGNIZED BY THE MH27 ANTIBODY presented in International C. elegans Meeting. Unpublished information; cite only with author permission.

    The antibody MH27 is extensively used for the staining of epithelial junctions in developmental studies of C.elegans. The antigen recognized by the antibody localizes to adherens junctions as shown by immunogold staining (David Hall, pers. comm.). The gene encoding the antigen for the MH27 antibody (provisionally called mh27) maps to the center of the X-chromosome. A putative cDNA clone (mh-1) had been isolated in collaboration with A. Radice (New York Blood Center) by probing a lgt11 expression library with the MH27 antibody. Its sequence corresponds to genomic sequence within the cosmid C25A11 and to the Kohara cDNA yk28c11. Sequencing of yk28c11 revealed a large open reading frame (about 1.4 kb) that shows structural homology to the vertebrate proteins trichohyalin and caldesmon. C25A11 is covered by the deficiency stDf5 and homozygous deficiency embryos do not stain with MH27 (Terns et al., 1997. Genetics, in press). Embryos injected with mh-1 anti-sense RNA have been shown to arrest at the 2-3 fold stage and showed either just patches of MH27 staining or no staining; occasionally there is staining of the pharynx. We are currently generating and testing mh27-GFP fusion constructs which could prove very helpful for studying epithelial morphogenesis. In order to further characterize mh27 we are attempting to isolate a mutation in the gene using a pre-complementation screen: We will look for embryonic lethality in the absence of an array containing C25A11. Also, we are preparing to perform Northern analysis of the mh27 transcript and to clone the complete transcript using RT-PCR. Acknowledgments: We would like to thank Ahna Skop for assistance with initial antisense injection experiments.

    Affiliation:
    - Zoology Research, University of Wisconsin, Madison, WI 53706 Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY 10021


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