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Resources » Paper

Boontrakulpoontatwee P et al. (1995) International C. elegans Meeting "UNUSUAL UNC-44 REVERTANTS CONTAIN ADDITIONAL TRANSPOSON INSERTIONS"

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  • Comments on Boontrakulpoontatwee P et al. (1995) International C. elegans Meeting "UNUSUAL UNC-44 REVERTANTS CONTAIN ADDITIONAL TRANSPOSON INSERTIONS" (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00021978

    Boontrakulpoontatwee P, & Otsuka AJ (1995). UNUSUAL UNC-44 REVERTANTS CONTAIN ADDITIONAL TRANSPOSON INSERTIONS presented in International C. elegans Meeting. Unpublished information; cite only with author permission.

    Mutations in unc-44 result in aberrant axon guidance [Hedgecock et al., Dev. Biol., 111, 158-170 (1985)]. The unc-44 neurological defects appear to result from alterations to a novel ankyrin (AO13 ankyrin), encoded by a ~14 kb mRNA [Otsuka et al., J. Cell Biol., in press]. AO13 ankyrin is distinct from the vertebrate ANK-2 and ANK-3 ankyrins. DNA alterations in transposon-induced alleles and their revertants were determined (see table below). A variant of Tc5 produces the defect in st200, while surprisingly, the revertant has lost the Tc5 and has gained two face-to-face Tc1 elements. This unusual situation is mirrored by the single Tc1 in rh1042, while its revertant contains an additional downstream Tc1 element. The second Tc1 is at the same splice acceptor junction as that in the st200 revertant, but in the opposite orientation. Because activity can be restored by a 2.5 kb coding region deletion in the mn339 revertant, it is hypothesized that the carboxyl terminal domain (or 3' UTR) is critical for activity, and that blockage of the splice acceptor in the st200 and rh1042 revertants results in restoration of gene activity by alternative splicing.


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