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Resources » Paper

Colledge CJ et al. (1995) International C. elegans Meeting "sem-2, a Gene Required for Muscle Development in the MS Lineage."

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  • Comments on Colledge CJ et al. (1995) International C. elegans Meeting "sem-2, a Gene Required for Muscle Development in the MS Lineage." (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00021707

    Colledge CJ, & Waterston RH (1995). sem-2, a Gene Required for Muscle Development in the MS Lineage presented in International C. elegans Meeting. Unpublished information; cite only with author permission.

    The MS lineage of C. elegans produces three distinct muscle types. During embryonic development the MS lineage gives rise to both body-wall and pharyngeal muscle and also to the M mesoblast (1). The M mesoblast goes on to divide and form 14 body-wall muscles and the 2 sex myoblasts duing post-embyonic development (2). The 2 sex myoblasts divide and form the 8 uterine and 8 vulval muscles. The first allele of sem-2 was identified as an Egl mutant. From polarized light microscopy it was determined that the Egl phenotype was caused by the absence of the sex muscles. Lineage analysis revealed that the 2 sex myoblasts had undergone a cell-fate specification change and had adopted the fate of body-wall muscles (M. Stern, personal communication). A non-complementation screen was initiated to identify new alleles of sem-2. From a screen of 45,000 genomes, 5 new alleles of sem-2 were isolated. 1 of the newly identified alleles results in a phenotype similar to the original sem-2 mutation. The other four alleles of sem-2 result in embryonic lethality with arrest of development occuring at the 1 1/2 fold stage. Mutant embryos fail to elongate their heads resulting in a bloated head appearance. Antibody staining revealed a lack of anterior body-wall muscles in the mutant embryos which is consistent with a failure of the MS lineage to produce body-wall muscle. Further antibody staining showed however that in the mutant embryos the MS lineage is still capable of producing pharyngeal muscles. We are currently in the process of trying to clone the sem-2 gene and further characterize the phenotype of sem-2 mutants.


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