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Resources » Paper

Feichtinger R et al. (1991) International C. elegans Meeting "A SCREEN FOR MATERNAL EFFECT LETHALS USING THE BALANCER nTl"

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00020759

    Feichtinger R, Schnabel H, & Schnabel R (1991). A SCREEN FOR MATERNAL EFFECT LETHALS USING THE BALANCER nTl presented in International C. elegans Meeting. Unpublished information; cite only with author permission.

    In the first 120 minutes of development of C. elegans, until the embryo consists of 28 cells, a great deal of determination is already achieved: the embryonic axes have been established, all embryonic founder cells have been generated, the monoclonally derived germline and gut have been segregated and the primary inductive events have already occurred. Most of the early decisions are thought to be maternally controlled. We are screening for maternal effect lethal mutations with the aim to saturate part of the genome. The balancer nTl is used in order to maintain nonconditional mutants as heterozygotes. With nTl, 60% of chromosome IV and 65% of chromosome V, according to the physical map, are balanced, accounting for V5 of the genome. We want to complement the mutations prior to phenotypic analysis in order to look at several independently isolated mutant alleles for each locus. Since complementation analysis raises in effort quadratically with the number of mutations, grouping by deficiency-mapping is very profitable. In our first set we obtained maternal effect lethals at a rate of 0.015 per haploid genome. We intend to isolate about 420 mutations (corresponding to 28000 haploid genomes). Using a statistical analysis of our data of a similar screen with mnCl we can calculate a projection for this screen. A negative binomial probability distribution is used to simulate the more frequently appearing loci. We estimate a mean saturation of 3 and a remarkably large number of 95 loci of this kind, i.e. maternal genes, in this region. Half of them would be represented with more than one mutant allele.


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