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Resources » Paper

Jonathan M Rhine et al. (2003) International Worm Meeting "Cloning pat-12, an atypical Pat gene that may be necessary for proper muscle attachment"

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  • Comments on Jonathan M Rhine et al. (2003) International Worm Meeting "Cloning pat-12, an atypical Pat gene that may be necessary for proper muscle attachment" (0)

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00019649

    Jonathan M Rhine, A Craig Mackinnon, & Benjamin D Williams (2003). Cloning pat-12, an atypical Pat gene that may be necessary for proper muscle attachment presented in International Worm Meeting. Unpublished information; cite only with author permission.

    Accurate and effective muscle function requires the transmission of forces via specific attachment complexes. In our lab, we study C. elegans mutants in which these attachments in the body wall muscles have been compromised in some way. These mutants together form a class whose mutants display share a common embryonic lethality termed the Pat (Paralyzed and Arrested at Two-Fold) phenotype. One such Pat mutant, pat-12, was mapped to linkage group III between unc-45 and daf-7. Further work suggested that the predicted gene T17H7.4 might correspond to pat-12. The predicted gene T17H7.4 encodes a complex involving as many as 11 splice isoforms and 25 exons and encompasses over 20 kB of genetic space. Previous published data by Gonzy et al. as well as our own data showed that T17H7.4 showed an embryonic lethal RNAi phenotype (100% and 96.2% respectively). Moreover, this lethal phenotype was the same as the previously described PAT phenotype. Rescue experiments using the cosmids containing the genetic region surrounding and containing T17H7.4 were able to rescue the Pat phenotype in pat-12(st430) mutant individuals. In addition, we were able to sequence a mutation in the pat-12(st430) line in the genetic region corresponding to T17H7.4. This G to A mutation leads to the loss of a splice acceptor site shared by 5 of the predicted slice isoforms. Preliminary staining using an antibody raised against PAT-12 stained circumferential rings overlying the body wall muscles.


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