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Resources » Paper

Tadao Inoue et al. (2003) International Worm Meeting "DAF-21/HSP90 functions during gonad development"

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00019620

    Tadao Inoue, Johji Miwa, Sonoko Kawano, & Yasunori Yamaguchi (2003). DAF-21/HSP90 functions during gonad development presented in International Worm Meeting. Unpublished information; cite only with author permission.

    HSP90 has been believed to act as a molecular chaperone and has been shown to be necessary for some developmental processes, such as signal transduction. The daf-21 (p673) mutant in C. elegans has been identified to carry a point mutation in the hsp90 homologue gene (Birnby, 2000).We have previously reported that RNA-mediated interference directed at daf-21 resulted in embryonic or larval lethality, reduced brood size, or/and sterility, which seemed to be caused by a germline defect (MidWest Worm Meeting, 2002). In addition to those, we revealed a novel aspect of DAF-21 function by daf-21 RNAi that impairment of DAF-21 function strongly affected the gonad morphology.In C. elegans, the hermaphrodite gonad consists of two U-shaped tubular arms extended anteriorly and posteriorly, each of which possesses a cell at the distal tip, called DTC. The arms are constructed in three distinct migration phases directed by the DTCs: (1) centrifugal phase, (2) ventral-to-dorsal phase, and (3) centripetal phase. More than 70% of daf-21 (RNAi) animals showed a gonadogenesis defect with a variety of DTC migration patterns in F1 and later generations. Many of these affected animals exhibited a precocious entry into the second DTC migration phase or an extra turning of the DTCs. Furthermore, others displayed no second migration phase, only the first and third phase, thus resulting in a ventral DTC reflex, which was similar to that seen in unc-5 mutants. It has been reported that unc-5 is required for the ventral-to-dorsal migration phase. Previous studies have shown that cell-autonomous unc5 expression in DTCs is necessary and sufficient for the second migration phase. Based on these phenotypic similarities with daf-21 (RNAi), an experiment to identify the timing of unc-5 expression by the testing of unc-5::gfp in daf-21 (RNAi) is underway. In addition, the daf-21 (p673) mutant strain exhibited a temperature sensitivity for the DTC migration at 25C. Taken together, our findings suggest that DAF-21 function may be required during gonad development.


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