Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Cheryl L Van Buskirk et al. (2003) International Worm Meeting "Do the splice variants of lin-3 differ in function?"

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Cheryl L Van Buskirk et al. (2003) International Worm Meeting "Do the splice variants of lin-3 differ in function?" (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00019465

    Cheryl L Van Buskirk, & Paul W Sternberg (2003). Do the splice variants of lin-3 differ in function? presented in International Worm Meeting. Unpublished information; cite only with author permission.

    The lin-3 gene encodes the only known ligand of the EGF receptor in C. elegans, and is required for viability, ovulation, vulval patterning, and other cell fate specifications. Two splice variants, lin-3A and 3B, which arise from alternate splice donor site usage, have been previously identified. Lin-3B differs from the more abundant lin-3A by the insertion of an extra 15 amino acids just N-terminal to the transmembrane domain. This region is known to be important for proteolytic processing in other EGF-like ligands. We have recently identified an additional variant, lin-3C, that arises from alternate exon usage and results in the insertion of 41 amino acids in this same juxtamembrane region. While lin-3A and lin-3B appear to be expressed at all stages of development, lin-3C is detected only during early larval stages. To determine the significance of alternative splicing within lin-3, we are testing the ability of each isoform to rescue the vulval patterning defects of a weak lin-3 mutant, and we are also examining the effect of heat shock-induced overexpression of each isoform on normal development.


    Tip: Seeing your name marked red? Please help us identify you.