Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Jun Liu et al. (2001) International C. elegans Meeting "Essential roles for C. elegans lamin gene in nuclear organization, cell cycle progression and spatial organization of nuclear pore complexes"

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Jun Liu et al. (2001) International C. elegans Meeting "Essential roles for C. elegans lamin gene in nuclear organization, cell cycle progression and spatial organization of nuclear pore complexes" (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00018158

    Jun Liu, Tom Rolef Ben-Shahar, Dieter Riemer, Millet Treinin, Perah Spann, Klaus Weber, Andrew Fire, & Yosef Gruenbaum (2001). Essential roles for C. elegans lamin gene in nuclear organization, cell cycle progression and spatial organization of nuclear pore complexes presented in International C. elegans Meeting. Unpublished information; cite only with author permission.

    We are interested in using C. elegans as a model system to study the function of nuclear lamin and lamin-associated proteins during development. C. elegans has a single lamin gene, designated lmn-1 (previously termed CeLam-1). Antibodies raised against the lmn-1 product (Ce-lamin) detected a 64 kD nuclear envelope protein. Ce-lamin was detected in the nuclear periphery of all cells except sperm and was found in the nuclear interior in embryonic cells and in a fraction of adult cells. Reductions in the amount of Ce-lamin protein produce embryonic lethality. Although the majority of affected embryos survive to produce several hundred nuclei, defects can be detected as early as the first nuclear divisions. Abnormalities include rapid changes in nuclear morphology during interphase, loss of chromosomes, unequal separation of chromosomes into daughter nuclei, abnormal condensation of chromatin, an increase in DNA content, and abnormal distribution of nuclear pore complexes (NPCs). Under conditions of incomplete RNA interference a fraction of embryos escaped embryonic arrest and continue to develop through larval life. These animals exhibit additional phenotypes including sterility and defective segregation of chromosomes in germ cells. Our observations show that lmn-1 is an essential gene in C. elegans , and that the nuclear lamins are involved in chromatin organization, cell cycle progression, chromosome segregation, and correct spacing of NPCs.

    Authors: Jun Liu, Tom Rolef Ben-Shahar, Dieter Riemer, Millet Treinin, Perah Spann, Klaus Weber, Andrew Fire, Yosef Gruenbaum


    Tip: Seeing your name marked red? Please help us identify you.