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Resources » Paper

Chen WI et al. (1996) West Coast Worm Meeting "THE CAENORHABDITIS ELEGANS P21-ACTIVATED KINASE (CEPAK) COLOCALISES WITH CERAC1 AND CDC42CE AT HYPODERMAL CELL BOUNDARIES DURING EMBRYO ELONGATION"

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  • Comments on Chen WI et al. (1996) West Coast Worm Meeting "THE CAENORHABDITIS ELEGANS P21-ACTIVATED KINASE (CEPAK) COLOCALISES WITH CERAC1 AND CDC42CE AT HYPODERMAL CELL BOUNDARIES DURING EMBRYO ELONGATION" (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00017244

    Chen WI, Chen S, & Lim L (1996). THE CAENORHABDITIS ELEGANS P21-ACTIVATED KINASE (CEPAK) COLOCALISES WITH CERAC1 AND CDC42CE AT HYPODERMAL CELL BOUNDARIES DURING EMBRYO ELONGATION presented in West Coast Worm Meeting. Unpublished information; cite only with author permission.

    The Ras-related Rho p21s mediate signaling pathway leading to cytoskeletal reorganization. One of such morphological changes in the nematode C. elegans development occurs during the embryonic body elongation characterized by the hypodermal cell fusion. To investigate the possible involvement of the targets of Rho p21s in this process, we have isolated the cDNA coding for the p21 Rac1/CDC42-activated kinase (CePAK) from a C. elegans embryonic cDNA library. This 2.35 kb cDNA encodes a polypeptide of 572 amino acid residues, with the highly conserved N-terminal p21-binding and the C-terminal kinase domains. Similar to its mammalian and Drosophila homologs, the CePAK protein expressed in E.coli exhibits binding activity towards GTP-bound CeRac1 and CDC42Ce. The polyclonal antibody recognizes a specific 70 kDa protein from embryonic extracts which displays CeRac1/CDC42Ce-binding and kinase activities. Immunofluorescence analysis indicates that CePAK is specifically expressed at the hypodermal cell boundaries during embryonic body elongation. Interestingly, CeRac1 and CDC42Ce are found at the same location as CePAK which might point to their common involvement in the hypodermal cell fusion, a crucial cytoskeletal reorganization process for nematode development.

    Affiliation:
    - Glaxo-IMCB Group, Institute of Molecular & Cell Biology, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Republic of Singapore Institute of Neurology, 1 Wakefield Street, London WC1N 1PJ, United Kingdom


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