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Resources » Paper

Clark DV et al. (1986) Worm Breeder's Gazette "Tc1-induced lethal mutations on LGIV (right) and LGV (left)."

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  • Comments on Clark DV et al. (1986) Worm Breeder's Gazette "Tc1-induced lethal mutations on LGIV (right) and LGV (left)." (0)

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    Status:
    Publication type:
    Gazette_article
    WormBase ID:
    WBPaper00016540

    Clark DV, McKim KS, & Baillie DL (1986). Tc1-induced lethal mutations on LGIV (right) and LGV (left). Worm Breeder's Gazette, 9(3), 31. Unpublished information; cite only with author permission.

    As part of our ongoing genetic analysis into the organization of essential genes on LGIV (right) and LGV (left), we have conducted a screen for Tc1-induced lethal mutations in these regions. A strain was constructed with the mutator activity on LGI (derived from RW7037, Moerman and Waterston, 1984, Genetics, 108:859-877) and the balancer, nT1 (Ferguson and Horvitz, 1985, Genetics, 110:170-72), which suppresses recombination on LGIV from almost unc-17 to dpy-4 and on LGV from unc-60 to at least sma-1 [approximately 40 m.u. overall). The genotype of the strain used for our screen was mut-4/mut-4 (I?;unc- 22/nT1(IV);unc-46/nT1(V)]. We screened 3503 F1 'chromosomes' and 35 lethal mutations were recovered: a rate of 1%. In order to reduce the likelihood of reversion or further Tc1-induced mutations, each strain was outcrossed and the 'mutator' chromosome was segregated out. This was done by using a dpy-5 marked chromosome [see Mori et al., WBG 9(1), p. 25]. So far, 7 lethals have mapped to LGIV and 23 to LGV. Comparison with EMS mutagenesis data [4.4% lethals on LGIV, 3.4% lethals on LGV; see WBG 9(2), p. 82] indicates that there is probably a mutational hotspot for Tc1 on LGV. The deficiency mapping and inter se complementation tests of these lethals, now in progress, will indicate if this is the case. Tc1-tagged essential genes will be extremely useful for characterization and positioning of cloned sequences with respect to the genetic maps of LGIV(right) and LGV(left).


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