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Resources » Paper

Ward S et al. (1983) Worm Breeder's Gazette "Monoclonal and Polyclonal Antibodies that Bind to Sperm Antigens"

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  • Comments on Ward S et al. (1983) Worm Breeder's Gazette "Monoclonal and Polyclonal Antibodies that Bind to Sperm Antigens" (0)

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    Status:
    Publication type:
    Gazette_article
    WormBase ID:
    WBPaper00016214

    Ward S, & Hogan E (1983). Monoclonal and Polyclonal Antibodies that Bind to Sperm Antigens. Worm Breeder's Gazette, 8(1). Unpublished information; cite only with author permission.

    We have examined a number of monoclonal and polyclonal antisera generously provided to us by other labs in order to assess their binding to sperm antigens. The antisera include the following: two rabbit sera and eight mouse monoclonals raised against muscle proteins by Ross Francis: five anti-sperm mouse monoclonals prepared by Susi Strome and obtained initially from Michael Klass; eight anti-sperm mouse monoclonals prepared by Fred Pavalko and Tom Roberts; and a rabbit anti-glyceraldehyde phosphate dehydrogenase (GAPDH) serum obtained from Patrice Yarborough and Ralph Hecht. Our interest in these sera is to use them to identify new sperm antigens whose cellular localization and developmental history can be compared with the sperm specific antigens we have alrealy examined. All the antisera were first assayed for tissue specificity by a solid phase immunoassay with proteins from sperm, eggs, larvae or fem- 1( hc17) hemaphrodites which have no sperm. Those antisera that showed significant binding to sperm proteins were then characterized further by immunostaining of sperm or fem-1 proteins transferred from 1-D or 2- D gels to nitrocellulose ('Western transfers'). In addition the cellular localization of antigens was determined by immunofluorescence.


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