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Resources » Paper

Lew KK et al. (1979) Worm Breeder's Gazette "frozen mutagenized worms remain a good source for mutant isolation."

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  • Comments on Lew KK et al. (1979) Worm Breeder's Gazette "frozen mutagenized worms remain a good source for mutant isolation." (0)

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    Status:
    Publication type:
    Gazette_article
    WormBase ID:
    WBPaper00015967

    Lew KK, & Miwa J (1979). frozen mutagenized worms remain a good source for mutant isolation. Worm Breeder's Gazette, 4(1), 15. Unpublished information; cite only with author permission.

    Mutagenesis is hazardous not only to animals to be mutagenized, but to ourselves as well. We, being carcinogen-/mutagen-phobics, have developed a procedure that would alleviate some hazard as well as phobia, by mutagenizing a large stock of N2 or other strains and freezing them away for future use. Strains to be mutagenized are grown on Petri plates until bacteria are nearly exhausted, that is, just before the end of the exponential growth phase. The worms are washed from the plates and treated with ethyl methanesulfonate (EMS) as described by Brenner (1974). The mutagenized worms free of mutagen are frozen in liquid N2. They are thawed out as needed. A good batch of mutagenized stocks can be permanently stored. Most survivors are younger than L3. Forward mutation rate, as measured by dumpy and uncoordinated worms, indicates that such survivors remain a good source of mutants. This procedure should also work with other mutagens.


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