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Comments on Pilgrim DB et al. (2000) Worm Breeder's Gazette "Maternal UNC-45 may have a role in early embryos independent of muscle function" (0)
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Pilgrim DB, & Ao W (2000). Maternal UNC-45 may have a role in early embryos independent of muscle function. Worm Breeder's Gazette, 16(2), 35. Unpublished information; cite only with author permission.
The unc-45 gene was one of the first ever reported in worms (Epstein and Thomson, 1974) yet we still do not have a satisfactory explanation of its role in development. Missense alleles at the locus show a temperature-sensitive disorganization of muscle thick filament structure, while nonsense alleles are lethal, with varying maternal effects. unc-45 reporter constructs show zygotic expression which is exclusive to muscle cells, and antibodies against the protein show a co-localization with MHC B, but not MHC A, in thick filaments. The phenotype of unc-45 (ts) alleles can be suppressed if all MHC B is removed, and MHC A overexpressed, as unc-45(ts);unc-54(0);myo-3(sup-3) animals are almost normal. This would suggest that the function of UNC-45 is restricted to some sort of MHC B-specific interaction.