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Resources » Paper

Chalfie M et al. (1999) Worm Breeder's Gazette "Dye-coupling in Wild-type C. elegans Neurons"

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    Status:
    Publication type:
    Gazette_article
    WormBase ID:
    WBPaper00015651

    Chalfie M, Goodman MB, & Lockery SR (1999). Dye-coupling in Wild-type C. elegans Neurons. Worm Breeder's Gazette, 16(1), 45. Unpublished information; cite only with author permission.

    Small, fluorescent dyes permeate gap junctions between neurons in both vertebrates and invertebrates. While not all gap junctions are dye-permeable, dyes are useful tools for determining the extent of coupling among neurons. We have tested the ability of 3 low molecular weight dyes to permeate gap junctions between neurons in worms. We focused on dyes that could be visualized simultaneously with GFP (Table I). All 3 dyes labeled multiple cell bodies when introduced via a patch pipette into a single cell body, indicating that these dyes could permeate neuronal gap junctions. To date, the largest group of dye-coupled cell bodies had only 3 labeled cells. This observation suggests that functional coupling may be restricted to first-order partners, despite an extensive network of gap junctions between neurons.


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