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Comments on Bakaev VV (1998) Worm Breeder's Gazette "AN ATTEMPT TO SLOW AGING IN C. elegans. 7. NO EFFECT OF SODIUM ADENOSINETRIPHOSPHATE" (0)
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Bakaev VV (1998). AN ATTEMPT TO SLOW AGING IN C. elegans. 7. NO EFFECT OF SODIUM ADENOSINETRIPHOSPHATE. Worm Breeder's Gazette, 15(2), 56. Unpublished information; cite only with author permission.
The purpose of this study was to investigate the effect of different concentrations of sodium adenosinetriphosphate in water solutions on nematode life span. In this experiment sodium adenosinetriphosphate was used in following dilutions 1:10*3, 1:10*4, 1:10*5, 1:10*6 and 1:10*7. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without sodium adenosinetriphosphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with sodium adenosinetriphosphate in any concentration) every day (one worm in one well). This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Concentration of Longevity (days) sodium adenosinetriphosphate n mean +/- S.E. maximal Control 12 20.8 +/- 1.7 27 1:10*3 12 5.6 +/- 0.2 6 1:10*4 12 6.0 +/- 0.2 8 1:10*5 12 10.7 +/- 1.2 20 1:10*6 12 20.7 +/- 1.3 27 1:10*7 12 20.9 +/- 1.8 26
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