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Resources » Paper

Scamps C et al. (1996) Worm Breeder's Gazette "HERE IS HIR"

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    Status:
    Publication type:
    Gazette_article
    WormBase ID:
    WBPaper00015268

    Scamps C, Thierry-Mieg D, & Lipinski M (1996). HERE IS HIR. Worm Breeder's Gazette, 14(2), 86. Unpublished information; cite only with author permission.

    The Hir protein family comprises HIR1 and HIR2, two proteins identified in yeast as histone transcriptional regulators, and the product of the HIRA gene cloned from the chromosome 22 region critical for the DiGeorge syndrome, a human haploinsufficiency disease affecting derivatives of the third and fourth pharyngeal pouches (Lamour et al., Hum. Mol. Genet. 1995, 5: 731-739). These WD repeat-containing proteins appear to play a role in nuclear architecture as part of multiprotein complexes. We have identified a family member through screening of the C. elegans database. The corresponding gene, K10D2.1, maps to chromosome III, between the cloned genes ced-4 and clk-1. Using primers from the 3' end of the yk22d7 cDNA (kindly provided by Yuji Kohara), we have shown by Southern blotting that a unique gene in C. elegans hybridizes to our .2 kb probe and have designed a PCR assay to test for the presence of K10D2.1 in the deficiencies of the area, kindly provided by David Baillie. To our surprise, the gene is present in the three deficiencies sDfl30, sDfl21 and sDf134, indicating that it maps left of their left limits. We propose below a revised map in which all the genetic and molecular data is consistently taken into account. To identify a strain with a mutation in the C. elegans HIR gene, the K10D2 cosmid will be tested for its ability to rescue selected mutants.

    Affiliation:
    - Institut Gustave Roussy, Villejuif, France CNRS-CRBM, Montpellier, France


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