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Maduro MF, & Pilgrim DB (1996). eDf2 is not a terminal deletion. Worm Breeder's Gazette, 14(2), 28. Unpublished information; cite only with author permission.
The LGIII-derived aberrations eDf2 and eDp6 were obtained concomitantly after acetaldehyde mutagenesis(1). The left-hand eDf2 portion (approximately two-thirds of LGIII) behaves genetically like a terminal deletion, and the remaining portion of LGIII formed the free duplication eDp6, which may be circular (Hunter and Wood, WBG 10#2, p.150). eDf2 complements mutations to the left of unc-119, and eDp6 complements all those to the right. We have previously shown that animals homozygous for eDf2 and eDp6 display the same phenotype as unc-119 mutants, and that eDf2; eDp6 animals transgenic for unc-119 rescuing clones are indistinguishable from wild type, consistent with unc-119 being the only gene affected(2). It is of interest to understand the nature of these aberrations, perhaps as a model for understanding the healing of chromosome breaks in C. elegans. Our previous long-range restriction analysis has shown that there are at least 100 kbp of DNA that have been added beyond the eDf2 breakpoint (WBG 13#2, pp.64-65), suggesting that the healing of the break involved the addition of a large amount of DNA, perhaps from the original right end of LGIII, either as a direct splice or as a result of a recombination event.
Affiliation:
- Dept. of Biological Sciences, University of Alberta, Edmonton, Alberta CANADA T6G 2E9