Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Driscoll MA et al. (1988) Worm Breeder's Gazette "notes on the DNA sequence of most of the ben-1 gene."

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Driscoll MA et al. (1988) Worm Breeder's Gazette "notes on the DNA sequence of most of the ben-1 gene." (0)

  • Overview

    Status:
    Publication type:
    Gazette_article
    WormBase ID:
    WBPaper00013972

    Driscoll MA, Bergholz E, & Chalfie M (1988). notes on the DNA sequence of most of the ben-1 gene. Worm Breeder's Gazette, 10(3), 41. Unpublished information; cite only with author permission.

    The ben-1 gene encodes one of the four (or so) C. elegans - tubulins. All mutations that confer resistance to the antimicrotubule drug benomyl map to this locus. The ben-1-encoded -tubulin is not essential for C. elegans coordination or viability since animals harboring deletions of the ben-1 gene appear wild-type in growth and behavior. We have sequenced all but the very 3' end of the ben-1 gene. 408 of approximately 450 amino acids are represented. (The sequence data is available upon request). In Genbank, the deduced protein sequence is most homologous to a human -tubulin (showing 93% identity, with all amino acid changes conservative). Comparison to other C. elegans - tubulins indicates that ben-1 is 96% identical to the tub-1 gene sequenced by L. Gremke and J. Culotti. ben-1 shares 87% identity with the mec-7-encoded -tubulin. The C. elegans -tubulin gene organization can be summarized as follows: [See Figure 1] ben-1 and tub-1 are similar in organization of coding regions, whereas mec-7 differs markedly in the relative positioning of exons and introns. The ben-1 gene contains two relatively long introns, of 973 and 809 bp. The putative splice sites in ben-1 conform to consensus C. elegans splice sequences. Interestingly, there is a potential splice acceptor site upstream of the AUG initiator codon. We are concerned that the sequenced 350bp 5' to the translational start may not include the promoter for this gene. Possibly, the message may acquire a trans- spliced leader. The DNA sequence of the 5' region in a ben-1 cDNA should clarify the transcript structure.


    Tip: Seeing your name marked red? Please help us identify you.