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The flu-1 mutants (previously denoted fl-I, M.G.G. 135 39-44) of C. elegans which have a bright autofluorescence of the gut were thought to have much reduced kynurenine hydroxylase activity. The kynurenine hydroxylase activity in the crude extracts of 5 alleles of flu-1 mutant have been measured and found to be less than 7% of the specific activity of N2. This activity cosediments with succinic dehydrogenase activity in equilibrium sucrose density centrifugation. This suggests that kynurenine hydroxylase activity is associated with mitochondria.