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Resources » Paper

Fukata M et al. (2002) Cell "Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170."

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  • Comments on Fukata M et al. (2002) Cell "Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170." (0)

  • Overview

    PMID:
    12110184
    DOI:
    10.1016/S0092-8674(02)00800-0
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00013097

    Fukata M, Watanabe T, Noritake J, Nakagawa M, Yamaga M, Kuroda S, Matsuura Y, Iwamatsu A, Perez F, & Kaibuchi K (2002). Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170. Cell, 109, 873-85. doi:10.1016/S0092-8674(02)00800-0

    Linkage of microtubules to special cortical regions is essential for cell polarization. CLIP-170 binds to the growing ends of microtubules and plays pivotal roles in orientation. We have found that IQGAP1, an effector of Rac1 and Cdc42, interacts with CLIP-170. In Vero fibroblasts, IQGAP1 localizes at the polarized leading edge. Expression of carboxy-terminal fragment of IQGAP1, which includes the CLIP-170 binding region, delocalizes GFP-CLIP-170 from the tips of microtubules and alters the microtubule array. Activated Rac1/Cdc42, IQGAP1, and CLIP-170 form a tripartite complex. Furthermore, expression of an IQGAP1 mutant defective in Rac1/Cdc42 binding induces multiple leading edges. These results indicate that Rac1/Cdc42 marks special cortical spots where the IQGAP1 and CLIP-170 complex is targeted, leading to a polarized microtubule array and cell polarization.

    Authors: Fukata M, Watanabe T, Noritake J, Nakagawa M, Yamaga M, Kuroda S, Matsuura Y, Iwamatsu A, Perez F, Kaibuchi K


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