Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Mintzer EA et al. (2002) FEBS Lett "Behavior of a photoactivatable analog of cholesterol, 6-photocholesterol, in model membranes."

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Mintzer EA et al. (2002) FEBS Lett "Behavior of a photoactivatable analog of cholesterol, 6-photocholesterol, in model membranes." (0)

  • Overview

    PMID:
    11801250
    DOI:
    10.1016/S0014-5793(01)03271-9
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00012983

    Mintzer EA, Waarts BL, Wilschut J, & Bittman R (2002). Behavior of a photoactivatable analog of cholesterol, 6-photocholesterol, in model membranes. FEBS Lett, 510, 181-4. doi:10.1016/S0014-5793(01)03271-9

    6-Photocholesterol, a new photoactivatable analog of cholesterol in which a diazirine functionality replaces the 5,6-double bond in the steroid nucleus, was used recently to identify cholesterol-binding proteins in neuroendocrine cells [Thiele, C., Hannah, M.J., Farenholz, F. and Huttner, W.B. (2000) Nat. Cell Biol. 2, 42-49], to track the distribution and transport of cholesterol in Caenorhabditis elegans [Matyash, V., Geier, C., Henske, A., Mukherjee, S., Hirsh, D., Thiele, C., Grant, B., Maxfield, F.R. and Kurzchalia, T.V. (2001) Mol. Biol. Cell 12, 1725-1736], and to probe lipid-protein interactions in oligodendrocytes [Simons, M., Kramer, E.M., Thiele, C., Stoffel, W. and Trotter, J. (2000) J. Cell Biol. 151, 143-154]. To determine whether 6-photocholesterol is a faithful mimetic of cholesterol we analyzed the ability of this probe, under conditions in which it is not photoactivated to a carbene, to substitute for cholesterol in two unrelated assays: (1) to condense 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine monomolecular films and (2) to mediate the fusion of two alphaviruses (Semliki Forest and Sindbis) with liposomes. The results suggest that this analog is a suitable photoprobe of cholesterol.


    Tip: Seeing your name marked red? Please help us identify you.