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Resources » Paper

Tadao Inoue et al. (2002) Japanese Worm Meeting "Environmental stresses affect the daf-21 (hsp90) mRNA level in Caenorhabditis elegans"

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  • Comments on Tadao Inoue et al. (2002) Japanese Worm Meeting "Environmental stresses affect the daf-21 (hsp90) mRNA level in Caenorhabditis elegans" (0)

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00011947

    Tadao Inoue, Youji Fukunaga, Johji Miwa, & Yasunori Yamaguchi (2002). Environmental stresses affect the daf-21 (hsp90) mRNA level in Caenorhabditis elegans presented in Japanese Worm Meeting. Unpublished information; cite only with author permission.

    The daf-21 (p673) gene has been reported to encode the HSP90 homologue in C. elegans (Birnby DA et al., 2000). Not only does heat shock induce hsp genes, but also other environmental stresses have been found to do so. In the pathway of stress response to heat shock, two heat shock proteins, DAF-21 and HSP70, have been reported to restrain the activity of the heat shock transcription factor 1 (HSF1), which binds to heat shock elements and promotes hsp genes. The loss-of-function analysis for DAF-21 and HSP70 showed increased induction of hsp genes, thus indicating that DAF-21 and HSP70 function as negative regulators of HSF1. We previously reported that the mutation in daf-21 resulted in a 1.5-fold overexpression of both daf-21 and hsp70 mRNA as shown by Northern blot analysis. Based on this observation, we now used RT-PCR analysis to quantify the mRNAs for both of these genes in N2 and Daf-21(p673) and confirmed the previous results. As a new strategy, not only hsp90 (daf-21) but also other hsp genes, such as hsp70, hsp60, and hsp16, were examined to establish a bioassay system by which we can detect and quantify chemical and physical stresses on C. elegans. We found that this group of hsp genes was induced in C. elegans exposed to chemicals such as Cadmium ion, alcohols, and aldehydes. We intend to develop this hsp assay system to be a general assay method to test, detect, and predict the toxicity of chemical compounds.


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