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Resources » Paper

Femke Simmer et al. (2002) European Worm Meeting "rrf-3, a C. elegans strain with increased sensitivity to RNAi"

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00011461

    Femke Simmer, Julie Ahringer, & Ronald H A Plasterk (2002). rrf-3, a C. elegans strain with increased sensitivity to RNAi presented in European Worm Meeting. Unpublished information; cite only with author permission.

    Recent analysis on the RNA dependent RNA polymerase (RdRP) like genes of C. elegans hinted that mutations in the rrf-3 gene cause increased sensitivity to RNAi (Sijen et al., 2001). We now studied the rrf-3 RNAi phenotype more extensively. We selected 80 clones from the RNAi feeding library and compared rrf-3 to wild type animals upon feeding with these clones. We looked at embryonic and postembryonic phenotypes. Of the 80 genes tested we found for rrf-3 48 genes that gave phenotypes whereas for wild type animals we found phenotypes for 25 genes. Several genes have more then one associated phenotype. In total we detect 74 phenotypes for rrf- 3 animals and 44 phenotypes for wild type animals. For example we could detect very clear postembryonic phenotypes for dpy-18, lin-1 and unc-73. In wild type animals neuronally expressed genes appear to be more difficult to interfere with. We found for several of these genes, such as unc-30 and unc-33, a phenotype upon feeding of the corresponding dsRNA to rrf- 3, whereas we did not find a phenotype in wild type. This shows that the rrf-3 mutant strain has a general enhancement in sensitivity to RNAi for genes in all categories.


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