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Resources » Paper

Petra Bjoerk et al. (2002) European Worm Meeting "A novel conserved rna-binding domain protein, Rbd-1, is essential and important for ribosome biogenesis"

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  • Comments on Petra Bjoerk et al. (2002) European Worm Meeting "A novel conserved rna-binding domain protein, Rbd-1, is essential and important for ribosome biogenesis" (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00011381

    Petra Bjoerk, Goeran Bauren, ShaoBo Jin, Yong-Guang Tong, Thomas R Buerglin, Ulf Hellman, & Lars Wieslander (2002). A novel conserved rna-binding domain protein, Rbd-1, is essential and important for ribosome biogenesis presented in European Worm Meeting. Unpublished information; cite only with author permission.

    Synthesis of the ribosomal subunits from pre-rRNA requires a large number of trans-acting proteins and snoRNPs to execute base modifications, RNA-cleavages and structural rearrangements. We have characterized a novel protein, RBD-1 (RNA Binding-Domain-1), that is involved in ribosome biogenesis. This protein contains six consensus RNA-binding domains and is conserved as to sequence, domain organization and cellular location from yeast to human. In the dipteran Chironomus tentans, RBD-1 (Ct-RBD-1), binds pre-rRNA in vitro and anti-Ct- RBD-1 antibodies repress pre-rRNA processing in vivo. Ct-RBD-1 is mainly located in the nucleolus in an RNA polymerase I transcription dependent manner, but is also present in discrete foci in the interchromatin and the cytoplasm. In cytoplasmic extracts, 20-30% of Ct-RBD-1 is associated with ribosomes and, preferentially with the 40S ribosomal subunit. cDNA clone yk417f6 (kind gift of Yuji Kohara) spans the whole open reading frame of C. elegans rbd-1. We have performed RNAi experiments using this cDNA and shown that this gene is essential in Caenorhabditis elegans. Animals arrest mainly at the L1 larval stage. Our data suggest that RBD- 1 plays a role in structurally coordinating pre-rRNA during ribosome biogenesis and that this function is conserved in all eukaryotes.


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