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Resources » Paper

Michael Hoffmann et al. (2002) European Worm Meeting "Identification of an AAA ATPase as an interacting partner of C.elegans DLG-1"

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  • Comments on Michael Hoffmann et al. (2002) European Worm Meeting "Identification of an AAA ATPase as an interacting partner of C.elegans DLG-1" (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00011337

    Michael Hoffmann, Christoph Segbert, Carin Theres, & Olaf Bossinger (2002). Identification of an AAA ATPase as an interacting partner of C.elegans DLG-1 presented in European Worm Meeting. Unpublished information; cite only with author permission.

    We have performed a yeast 2-hybrid screen for direct binding partners of C.elegans MAGUK protein DLG-1 [1], the homologue of Drosophila tumor suppressor gene discs large. By screening 2.3 x 106 transformants, with PDZ domains 1-3, we have isolated 32 cDNA clones, corresponding to 21 different genes. At present, putative interacting partners are analyzed using RNA mediated interference (RNAi). Among these clones, six cDNAs were found to encode fragments of a C.elegans AAA ATPase (ATPases associated with a variety of cellular activities). A typical PDZ-binding motif (ETAV) is found at the carboxy terminus. AAA ATPases play important roles in a variety of cellular activities including proteolysis, protein folding, membrane trafficking, cytoskeletal regulation, DNA replication and intracellular motility [2-4]. Depletion of the protein, using RNAi, results in viable offspring that show developmental arrest during early larval stages. Staining of RNAi embryos and larvae with a-DLG-1 and MH27 antibodies revealed no mislocalization, so far. We are currently analyzing the functional basis of the larval-arrest phenotype and further validate the interaction with DLG-1. To determine the localization of the AAA ATPase, we expect to raise antibodies. Preliminary results will be presented.


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