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Resources » Paper

Belinda M Jackson et al. (2004) East Coast Worm Meeting "Identification of Wnt Pathway Target Genes in C. elegans by Microarray Analysis"

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  • Comments on Belinda M Jackson et al. (2004) East Coast Worm Meeting "Identification of Wnt Pathway Target Genes in C. elegans by Microarray Analysis" (0)

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00010665

    Belinda M Jackson, & David M Eisenmann (2004). Identification of Wnt Pathway Target Genes in C. elegans by Microarray Analysis presented in East Coast Worm Meeting. Unpublished information; cite only with author permission.

    During the well studied process of vulva development in C. elegans , six epithelial cells choose one of three cell fates based on activation of the RTK/Ras and Notch pathways in the vulva precursor cells (VPC). Our laboratory previously showed that a Wnt signaling pathway is also instrumental in vulva cell specification. We have shown that Axin (PRY-1), APC (APR-1), beta-catenin (BAR-1) and TCF (POP-1) act to maintain expression of the Hox gene LIN-39 in the VPCs. To identify other putative Wnt pathway targets important for vulva formation we will use Affymetrix microarray analysis. We previously showed that a heat shock inducible D NT BAR-1 protein leads to over-activation of Wnt signaling in the VPCs, causing extra vulva cell fates [1] . We will compare the gene expression profiles of worms over-expressing this activated BAR-1 product with that of wild-type worms and look for genes consistently up or down regulated. The caveat of this brute force approach will be a global affect on Wnt targets, including those not exclusive to the VPCs. In order to address this, we will also apply a strategy previously used to identify muscle cell specific gene expression in C. elegans [2] . Using a flag-tagged poly-A binding protein driven by a VPC specific promoter element, we will generate an mRNA pool derived predominantly from the vulva precursor cells, and analyze this by microarray analysis. Progress on both of these approaches will be reported. 1. Gleason, J.E., H.C. Korswagen, and D.M. Eisenmann, Activation of Wnt signaling bypasses the requirement for RTK/Ras signaling during C. elegans vulval induction. Genes Dev, 2002. 16 (10): p. 1281-90. 2. Roy, P.J., et al., Chromosomal clustering of muscle-expressed genes in Caenorhabditis elegans. Nature, 2002. 418 (6901): p. 975-9.


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