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Resources » Paper

Shakes DC et al. (2000) East Coast Worm Meeting "CDC27, an Anaphase Promoting Complex subunit, is required for the metaphase to anaphase transition during meiosis"

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  • Comments on Shakes DC et al. (2000) East Coast Worm Meeting "CDC27, an Anaphase Promoting Complex subunit, is required for the metaphase to anaphase transition during meiosis" (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00010486

    Shakes DC, Miller A, Wille L, Golden A, Sadler PL, Schumacher JM, & Abdolrasulnia M (2000). CDC27, an Anaphase Promoting Complex subunit, is required for the metaphase to anaphase transition during meiosis presented in East Coast Worm Meeting. Unpublished information; cite only with author permission.

    The metaphase to anaphase (met-ana) transition in mitotic cells is controlled by the activity of the Anaphase Promoting Complex (APC). APC is an E3 ubiquitin ligase that targets proteins for destruction in order for sister chromatids to separate at anaphase. The substrates of APC include cyclins, anaphase inhibitors, and chromosome cohesins. The APC is composed of at least 12 subunits in S. cerevisiae; mutations in these genes result in metaphase arrest. Temperature sensitive mutations in 6 genes disrupt the met-ana transition during meiosis I in C. elegans embryos. These genes are mat-1, -2, -3 (for met-ana transition defective), emb-1, -27, & -30 (Miwa et al., 1980; Cassada et al., 1981; Furuta et al., 2000; Nishiwaki & Miwa, WBG 10; Golden et al., 1999 Int. WM). Homozygous mothers shifted to 25C produce broods of 1-cell arrested embryos in which the oocyte chromosomes congress and set up a meiotic spindle after fertilization. However, the first meiotic division fails to occur, anaphase is never observed, polar bodies are never extruded, and pronuclei never form. The sperm chromosomes remain condensed and the sperm centrosomes fail to nucleate microtubule asters. The emb-30 gene was recently shown to encode an apc4 homolog (Furuta et al., 2000) and emb-27 a cdc16 homolog (another APC subunit; Sadler & Shakes, pers. comm.). Genetic mapping of mat-1 places it on LG I near another APC subunit gene, cdc27. RNAi of this gene results in the production of 1-cell arrested embryos that fail to progress through meiosis I. We have sequenced this cdc27 gene from a number of mat-1 alleles and found mutations in the cdc27 coding region for each. Based on 2-cell embryo and L1 shift-up experiments, the 6 mat-1 alleles make up an alleleic series. Two alleles are >95% lethal even at 15C and are maintained as balanced heterozygotes. Another 2 alleles result in sterility when embryos or L1s are shifted to 25C. The last 2 alleles are maternal-effect embryonic lethal; these animals, when shifted to 25C as 2-cell embryos, develop normally and produce 1-cell arrested embryos. We will show the sequence alterations and the phenotypic characterization of these 6 mat-1 alleles.


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