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Resources » Paper

Stone S et al. (1993) Gene "A Caenorhabditis elegans act-4::lacZ fusion: use as a transformation marker and analysis of tissue-specific expression."

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  • Comments on Stone S et al. (1993) Gene "A Caenorhabditis elegans act-4::lacZ fusion: use as a transformation marker and analysis of tissue-specific expression." (0)

  • Overview

    PMID:
    8406009
    DOI:
    10.1016/0378-1119(93)90290-J
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00001850

    Stone S, & Shaw JE (1993). A Caenorhabditis elegans act-4::lacZ fusion: use as a transformation marker and analysis of tissue-specific expression. Gene, 131, 167-73. doi:10.1016/0378-1119(93)90290-J

    A plasmid vector that serves as a dominant marker for isolating transformed animals in Caenorhabditis elegans has been constructed as a translational fusion of the C. elegans act-4 gene (encoding actin) and the Escherichia coli lacZ gene. This gene fusion can be used as a marker in transformation rescue experiments in any fertile strain of C. elegans. Progeny of animals injected with the act-4=lacZ fusion vector are stained histochemically with XGa1, and transformants turn blue. The internal eggs of stained animals remain viable, allowing recovery of the transformed strain. When the act-4=lacZ vector is co-injected with an unselected plasmid with which it shares some sequence homology, most transformants that are recovered by screening for expression of the act-4=lacZ fusion contain both plasmids. Production of active betaGal in animals transformed with the act-4=lacZ gene fusions appears to be limited to certain tissues. A chimeric gene that contains the 5' and 3' regions of act-4 is expressed strongly in the body-wall muscles, vulval muscles, and spermathecae. Addition of the internal portion of act-4, including the protein-coding region and introns, to this chimeric gene leads to additional lacZ expression in the pharynx.


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