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Resources » Paper

Rand JB et al. (1984) Genetics "Choline acetyltransferase-deficient mutants of the nematode Caenorhabditis elegans."

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  • Comments on Rand JB et al. (1984) Genetics "Choline acetyltransferase-deficient mutants of the nematode Caenorhabditis elegans." (0)

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    PMID:
    6698395
    DOI:
    10.1093/genetics/106.2.227
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00000675

    Rand JB, & Russell RL (1984). Choline acetyltransferase-deficient mutants of the nematode Caenorhabditis elegans. Genetics, 106, 227-48. doi:10.1093/genetics/106.2.227

    We have identified five independent allelic mutations, defining the gene cha-1, that result in decreased choline acetyltransferase (ChAT) activity in Caenorhabditis elegans. Four of the mutant alleles, when homozygous, lead to ChAT reductions of greater than 98%, as well as recessive phenotypes of uncoordinated behavior, small size, slow growth and resistance to cholinesterase inhibitors. Animals homozygous for the fifth allele retain approximately 10% of the wild- type enzyme level; purified enzyme from this mutant has altered Km values for both choline and acetyl-CoA and is more thermolabile than the wild-type enzyme. These qualitative alterations, together with gene dosage data, argue that cha-1 is the structural gene for ChAT. cha-1 has been mapped to the left arm of linkage group IV and is within 0.02 map unit of the gene unc-17, mutant alleles of which lead to all of the phenotypes of cha-1 mutants except for the ChAT deficiency. Extensive complementation studies of cha-1 and unc-17 alleles reveal a complex complementation pattern, suggesting that both loci may be part of a single complex

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