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Comments on Ohba K et al. (1982) Nematologica "A factor inducing dauer juvenile formation in C. elegans." (0)
Overview
Ohba K, & Ishibashi N (1982). A factor inducing dauer juvenile formation in C. elegans. Nematologica, 28, 318-325. doi:10.1163/187529282X00367
Caenorhabditis elegans were induced to form true dauer juveniles by the material lyophilized from a water solution in which living C. elegans (1 g fresh weight/50 ml distilled water) were incubated for 24 hr at 20C. The material best induced dauer formation at 23C, pH 6.0, 2% agar with 1/8 strength of Sayre et al.'s axenic medium. Overcrowding itself exerted no major effect. First and 2nd stage juveniles most actively secreted the dauer inducing substance into the incubating medium. Neither dauer juveniles nor other nematodes such as Bursaphelenchus xylophilus or Neoaplectana carpocapsae secreted the active substance.
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