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Resources » Paper

Edwards MK et al. (1983) Dev Biol "Location of specific messenger RNAs in Caenorhabditis elegans by cytological hybridization."

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  • Comments on Edwards MK et al. (1983) Dev Biol "Location of specific messenger RNAs in Caenorhabditis elegans by cytological hybridization." (0)

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    PMID:
    6343161
    DOI:
    10.1016/0012-1606(83)90094-5
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00000633

    Edwards MK, & Wood WB (1983). Location of specific messenger RNAs in Caenorhabditis elegans by cytological hybridization. Dev Biol, 97, 375-90. doi:10.1016/0012-1606(83)90094-5

    We have developed an autoradiographic technique for detecting specific Caenorhabditis elegans messenger RNA molecules in situ by hybridization of labeled, cloned DNA probes to fixed tissue sections and squashes of embryos and adults. We report analyses with probes of actin and collagen gene sequences from a C. elegans genomic clone library. Hybridization is RNase sensitive and tissue specific. In adults the actin probe, which recognizes cytoplasmic as well as muscle actin mRNA, hybridizes strongly to muscle and distal gonad (ovary), somewhat less strongly to maturing oocytes, and weakly to intestine. The collagen probe hybridizes weakly to distal gonad and intestine and very strongly to subcuticular tissues, in particular to the hypodermal cells and syncytial cytoplasm of the lateral hypodermal ridges, which are the sites of cuticle synthesis. In embryos, hybridization to squashes indicates that actin message is present at fertilization, decreases during early cleavage, and then increases again during morphogenesis. By contrast, collagen message is absent until the 100-cell stage and then increases rapidly during morphogenesis. The number of cells labeled is consistent with the view that the collagen probe hybridizes to hypodermal precursor cells. We estimate that our present methods can detect messages representing about 0.2% or more of the total mRNA population, and increases in this sensitivity should be possible. Therefore, the cytological hybridization technique should be useful for determining temporal and spatial patterns of specific mRNA distributions during development, at least for abundant and

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