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Resources » Paper

Hansen EL et al. (1970) Proc. Society for Experimental Biology & Medicine "Large scale preparation of liver growth factor for cultivation of nematodes."

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  • Comments on Hansen EL et al. (1970) Proc. Society for Experimental Biology & Medicine "Large scale preparation of liver growth factor for cultivation of nematodes." (0)

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    PMID:
    5480013
    DOI:
    10.3181/00379727-135-35080
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00000432

    Hansen EL, & Perez-Mendez G (1970). Large scale preparation of liver growth factor for cultivation of nematodes. Proc. Society for Experimental Biology & Medicine, 135, 487-489. doi:10.3181/00379727-135-35080

    Cultivation of metazoa under bacteria-free conditions is valuable for biochemical and nutritional studies and avoids the complexity introduced by associated organisms. For such studies the nematode has particular advantages. Investigation of the nutritional requirements of the free-living nematode Caenorhabditis briggsae led to the development of a chemically defined medium which required addition of a low level of an organic supplement to obtain continued reproduction. One very effective supplement is a partially purified protein designated growth factor (GF). This has been used in the culture of free-living nematodes and parasitic helminths. GF was originally prepared by a slow, laborious, stepwise elution from hydroxylapatite columns. In the present paper a rapid, efficient "batch" technique is described. Using this new method, batches yielding more than 2 g of GF have been prepared easily in a day.

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