Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Vanfleteren JR (1978) Annual Review of Phytopathology "Axenic culture of free-living, plant-parasitic, and insect-parasitic nematodes."

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Vanfleteren JR (1978) Annual Review of Phytopathology "Axenic culture of free-living, plant-parasitic, and insect-parasitic nematodes." (0)

  • Overview

    DOI:
    10.1146/annurev.py.16.090178.001023
    Status:
    Publication type:
    Review
    WormBase ID:
    WBPaper00000380

    Vanfleteren JR (1978). Axenic culture of free-living, plant-parasitic, and insect-parasitic nematodes. Annual Review of Phytopathology, 16, 131-157. doi:10.1146/annurev.py.16.090178.001023

    Two excellent reviews, one of them recent, are available on the axenic culture of nematodes. Therefore, the present article is mainly an outline of the present state of nematode culture research and is limited to surveying reports that have a significant bearing on our understanding of nematode growth in axenic culture. The first section covers nutritional requirements with special attention to the material needed for growth and reproduction in repeated subculture. Since nematode nutrition has largely been studied on the free-living nematode Caenorhabditis briggsae, I first scrutinize the dietary requirements of this species and extend our knowledge to other nematodes in axenic culture thereafter. In particular, I demonstrate how a general understanding of the nature of the growth factor emerges from a discriminatory analysis of existing knowledge and I support this concept with experimental evidence; also I attempt to reconcile this concept with some older as well as more recent controversial findings. The next section is devoted to culture methodology and covers some new techniques that render nematodes convenient as model organisms for scientific research. Throughout this review I use the terminology proposed by Dougherty for describing culture conditions with respect to the number of coexisting species and the different degrees of chemical differentiation of the media. The term axenic means free of contaminating organisms and supersedes the less suitable adjectives sterile or aseptic. Monoxenic cultures contain one additional species or tissue (e.g. alfalfa callus tissue), dixenic two and so on. Species that are studied in xenic cultures are reared in interaction with several unidentified organisms. Media that are chemically undefined are called oligidic. Those consisting of a defined basal portion and a less defined supplement are meridic. Holidic media are fully defined and contain

    Keywords:
    Tip: Seeing your name marked red? Please help us identify you.