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Resources » Paper

Dougherty EC et al. (1956) Proc. Society for Experimental Biology & Medicine "Axenic cultivation of Caenorhabditis briggsae (Nematoda: Rhabditidae). V. Maturation on synthetic media."

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  • Comments on Dougherty EC et al. (1956) Proc. Society for Experimental Biology & Medicine "Axenic cultivation of Caenorhabditis briggsae (Nematoda: Rhabditidae). V. Maturation on synthetic media." (0)

  • Overview

    PMID:
    13379469
    DOI:
    10.3181/00379727-93-22714
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00000102

    Dougherty EC, & Hansen EL (1956). Axenic cultivation of Caenorhabditis briggsae (Nematoda: Rhabditidae). V. Maturation on synthetic media. Proc. Society for Experimental Biology & Medicine, 93, 223-227. doi:10.3181/00379727-93-22714

    The free-living, soil-dwelling, hermaphroditic rhabditid nematode, C. briggsae, can mature under axenic conditions on a variety of media containing ingredients of complex, undefined composition-for example, certain liver fractions, plasma protein fractions, chick embryo juice, and extracts of equine pancreas, thymus, or spleen. Some of these media have also contained a large number of added known substances, but, until recently, all have included at high levels certain of the complex, crude substances mentioned. However, we have recently recorded success in rearing C. briggsae from larva to larva on a defined medium containing only a trace of the standard liver medium used by us in maintenance of stock axenic cultures of this organism. Now we are able to describe limited success following elimination of even this trace, such that C. briggsae can be reported as passing one generation on certain media of synthetic composition.

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