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[
Worm Breeder's Gazette,
1994]
The C. elegans genome sequencing project: A progress report. The C. elegans Genome Consortium, Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri, USA and Sanger Centre, Hinxton Hall, Cambridge, UK.
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[
Worm Breeder's Gazette,
1994]
The C. elegans genome sequencing project: A progress report. The C. elegans Genome Consortium, Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri, USA and Sanger Centre, Hinxton Hall, Cambridge, UK.
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[
Worm Breeder's Gazette,
1994]
Cytology of degenerin-induced cell death in the PVM neuron David H. Hall, Guoqiang Gu+, Lei Gong#, Monica Driscoll#, and Martin Chalfie+, * Dept. Neuroscience, Albert Einstein College of Medicine, Bronx, N.Y. 10461 + Dept. Biological Sciences, Columbia University, New York, N.Y. 10027 # Dept. Molecular Biology and Biochemistry, Rutgers University, Piscataway, N.J. 08855
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[
Worm Breeder's Gazette,
1998]
The purpose of this study was to investigate the effect of different concentrations of sodium adenosinetriphosphate in water solutions on nematode life span. In this experiment sodium adenosinetriphosphate was used in following dilutions 1:10*3, 1:10*4, 1:10*5, 1:10*6 and 1:10*7. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without sodium adenosinetriphosphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with sodium adenosinetriphosphate in any concentration) every day (one worm in one well). This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Concentration of Longevity (days) sodium adenosinetriphosphate n mean +/- S.E. maximal Control 12 20.8 +/- 1.7 27 1:10*3 12 5.6 +/- 0.2 6 1:10*4 12 6.0 +/- 0.2 8 1:10*5 12 10.7 +/- 1.2 20 1:10*6 12 20.7 +/- 1.3 27 1:10*7 12 20.9 +/- 1.8 26
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[
Worm Breeder's Gazette,
1998]
There are data on the life span prolonging effect of pineal peptide preparation epithalamin in mice, rats and Drosophila melanogaster [1,2]. The purpose of this study was to investigate the effect of different concentrations of epithalamin in water solutions on nematode life span. In this experiment epithalamin was used in following dilutions 1:10*5, 1:10*6, 1:10*7, 1:10*8, 1:lO*9 and 1:10*10. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without epithalamin) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with epitalamin in any concentration) every day (one worm in one well). This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Table I Concentration of Longevity (days) epithalamin n mean +/- S.E. maximal Control 12 17.4 +/- 2.5 29 1:10*5 12 12.8 +/- 2.1 27 1:10*6 12 15.1 +/- 1.9 28 1:10*7 12 17.1 +/- 2.2 28 1:10*8 12 19.6 +/- 2.2 29 1:10*9 12 18.8 +/- 2.0 31 1:10*10 12 18.7 +/- 1.7 25
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[
Worm Breeder's Gazette,
1999]
the effect of different concentrations of procaine hydrochloride in water solutions on nematode life span. In this experiment procaine hydrochloride was used in following dilutions: 1:10^3, 1:10^4, 1:10^5 and 1:10^6. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without procaine hydrochloride) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with procaine hydrochloride in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Concentration of procaine hydrochloride n Longevity (days) Mean + S.E. Maximal Control 24 9.7 + 0.6 15 1:10^3 24 11.3 + 0.8 20 1:10^4 24 11.3 + 1.0 25 1:10^5 24 14.3 + 1.9 38 1:10^6 24 11.2 + 0.9 21 Conclusion: If procaine hydrochloride solution was applied to C. elegans, it was able to increase their mean longevity [by 47.4 per cent, statistically significant (P < 0.05)] in dilution 1:105. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.
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[
Worm Breeder's Gazette,
1997]
The purpose of this study was to investigate the effect of different concentrations of pineal indole hormone melatonin in water solutions on nematode life span. Melatonin was used in following dilutions: 1:10*3, 1:10*4, 1:10*5, 1:10*6, 1:10*7, 1:10*8, 1:10*9, and 1:10*10. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without melatonin) during 4 hours, then they were transferred in next wells (with melatonin in any concentration) every day (one worm in one well). This investigation was carried out at temperature +21C and in darkness. The obtained results are presented in the table. Concentration of n Longevity (days) Melatonin Mean +/- S.E. Maximal Control 12 23.7 +/- 1.8 32 1:10*4 12 18.0 +/- 2.3 31 1:10*5 12 22.5 +/- 2.2 32 1:10*6 12 16.3 +/- 2.0# 27 1:10*7 12 15.6 +/- 2.4# 31 1:10*8 12 10.5 +/- 0.9# 14 1:10*9 12 11.3 -/- 1.6# 21 1:10*10 12 12.7 +/- 1.3# 22 #: The difference with control is significant, p Conclusion: If the water solution of melatonin was applied to C. elegans during the whole life span in above described conditions, it was not able to prolong the life span of these animals. In concentrations from 10*6 to 10*10 melatonin reduced the survival of nematoda. Acknowledgment: The authors wish to express their thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.
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[
Worm Breeder's Gazette,
2001]
The purpose of this study was to investigate the effect of different concentrations of sodium fusidine in water solutions on nematode life span. In this experiment sodium fusidine was used in following dilutions: 1:10 3 ,1:10 4 ,1:10 5 ,1:10 6 ,1:10 7 and 1:10 8 . Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and without sodium fusidine) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with sodium fusidine in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +21 0 C and in the darkness. The obtained results are presented in the following table. Concentration of sodium fusidine n Longevity (days) Mean +/- S.E. Maximal Control 36 9,47 +/- 0,76 21 1:10 3 36 10,03 +/- 0,81 23 1:10 4 36 9,44 +/- 0,88 25 1:10 5 36 8,72 +/- 0,51 18 1:10 6 36 9,86 +/- 0,94 26 1:10 7 36 8,86 +/- 0,73 24 1:10 8 36 8,86 +/- 0,73 15 Conclusion: If sodium fusidine solution was applied to C. elegans, it was not able to increase their mean as well as maximal longevity in comparison with control. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.
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[
Worm Breeder's Gazette,
1994]
THE ANNEXINS OF C. ELEGANS: A NOVEL SPLICE VARIANT MAY BE SUBJECT TO CONTROL BY PHOSPHORYLATION C.E Creutz. S.E Snyder. and S N. Daiele Dept. of Pharmacology, University of Virginia, Charlottesville, VA 22908 The annexins are calcium-dependent, phospholipid binding proteins that may function in exocytosis, membrane structure and permeation, and signal transduction. In order to establish a system for genetic analysis of their hypothetical functions, we have been characterizing the annexins of the nematode, C. elegans. Annexins were isolated from postmicrosomal supernatants by calcium dependent binding to lipid vesicles. EGTA extracts of these vesicles appear to contain primarily a single protein of mass 32kDa when examined by SDS-PAGE. Peptides derived from this protein were sequenced and verified that the worm protein is an annexin. An antiserum was raised to the worm annexin and was found to react almost exclusively with a 32kDa band in Western blots of worm homogenates. The antiserum was used to isolate cDNAs for the protein from a lambda gtll library (kindly provided by A, Fire). The sequence of the worm annexin is 42Z identical to that of bovine annexin IV, the closest vertebrate homolog. The single annexin gene was physically mapped to the vicinity of
dpy-17 in LGIII. One of three cDNA clones isolated contained a 15 base splice insertion encoding five amino acids near the N-terminus. This insertion contained THR and TYR residues that align exactly with phosphorylation sites in mammalian annexins for protein kinase C and the src kinase.
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[
Worm Breeder's Gazette,
2002]
The purpose of this study was to investigate the effect of different concentrations of streptomycin sulphate in presence of ascorbic acid (concentration 1:10 4 ) in water solutions on the nematode life span in reproductive period. In this experiment streptomycin sulphate was used in following dilutions: 1:10 1 , 1:10 2 , 1:10 3 , 1:10 4 , 1:10 5 , 1:10 6 and 1:10 7 . Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without ascorbic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without streptomycin sulphate in medium) every day (one worm in one well) beginning from third day. Then, from 3th to 10th day, these worms were transferred every day in next wells containing medium with streptomycin sulphate in any concentration. This investigation was carried out in temperature +21 deg C and in the darkness. The obtained results are presented in the following table. Concentration of streptomycin sulphate n Longevity (days) Mean +/- S.E. Maximal Control 12 12.8 +/- 1.1 18 1:10 3 12 13.7 +/- 0.7 21 1:10 4 12 14.0 +/- 0.6 23 Conclusion: If streptomycin sulphate solution in presence of ascorbic acid was applied to C. elegans , it was not able to increase significantly (P>0.05) their mean longevity in comparison with control. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.