-
[
Worm Breeder's Gazette,
1995]
It is well known that low temperatures can prolong longevity of different animals. In this study the experimental worms were mantained in liquid medium with E. coli in +21 C during the day (8-20 hrs) and in +4 C during the night, in darkness. One control group was mantained in +21 C and other control group was mantained in +4 C constantly. The obtained results are presented in the following table. ......................................................................... Control group Experimental Control group (+21C) group (+4C) Mean +/- S.D. Mean +/- S.D. Mean +/- S.D. .......................................................................... Mean longevity (days) 19,86 +/- 1,63 22,96 +/- 1,57 38,30 +/- 2,72 (n = 22) (n = 24) (n = 22) Maximal longevity (days) 34 35 50 Minimal longevity (days) 6 10 5 Mean fecundity 76,91 +/- 4,54 54,33 +/- 3,32 4,45 +/- 2,07 (n = 22) (n = 24) (n = 22) Maximal fecundity 118 95 46 Minimal fecundity 33 25 0 .................................................................... It can be concluded that such intermittent temperature is not able to prolong the life-span of C. elegans significantly, in comparison with constant cold, as well as fecundity. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (wild line) and E. coli OP50.
-
[
Worm Breeder's Gazette,
1995]
Three adult animals were kept in microtitre wells containing 0,75 ml of liquid medium with E. coli during 12 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). After second day number of progeny was calculated, if any animal during 2 days stopped reproduction, I used new medium with ethanol in different concentrations: 0,0% (control), 0,5%, 0% and 2,0%, 12 nematodes with every ethanol concentration. .............................................................. Ethanol concentration 0,0% 0,5% 1,0% 2,0% Mean life span (days) 23,6+/-1,8 20,1+/-1,0 19,5+/-0,9 22,0+/-1,4 Mean life span after ethanol addition (days) - 5,4+/-0,9 3,8+/-0,6 5,2+/-1,5 Number of progeny from 85,7+/-7,9 85,2+/-6,4 92,5+/-10,2 103,3+/-12,3 single worm Maximal number of progeny from one worm after - 35 44 60 ethanol addition .............................................................. It can be concluded, that ethanol has no effect on life span of aged C. elegans, but the fecundity slightly increased with increasing ethanol concentration. In 7 cases ethanol renewed reproduction in C. elegans after 2 day ceasing , although there was no life prolongation. Acknowledgment: The author wishes to express his gratitude to CGC for providing C. elegans (Bristol, N2, wild line) and E. coli OP50.
-
[
Worm Breeder's Gazette,
1998]
The purpose of this study was to investigate the effect of different concentrations of sodium adenosinetriphosphate in water solutions on nematode life span. In this experiment sodium adenosinetriphosphate was used in following dilutions 1:10*3, 1:10*4, 1:10*5, 1:10*6 and 1:10*7. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without sodium adenosinetriphosphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with sodium adenosinetriphosphate in any concentration) every day (one worm in one well). This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Concentration of Longevity (days) sodium adenosinetriphosphate n mean +/- S.E. maximal Control 12 20.8 +/- 1.7 27 1:10*3 12 5.6 +/- 0.2 6 1:10*4 12 6.0 +/- 0.2 8 1:10*5 12 10.7 +/- 1.2 20 1:10*6 12 20.7 +/- 1.3 27 1:10*7 12 20.9 +/- 1.8 26
-
[
Worm Breeder's Gazette,
2000]
The purpose of this study was to investigate the effect of different concentrations of rifampicin in water solutions on nematode life span. In this experiment rifampicin was used in following dilutions: 1:104, 1:105, 1:106, 1:107, 1:108, 1:109 and 1:1010. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and without rifampicin) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with rifampicin in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +210C and in the darkness.
-
[
Worm Breeder's Gazette,
2000]
The purpose of this study was to investigate the effect of different concentrati ons of streptomycin sulphate in water solutions on nematode life span. In this e xperiment streptomycin sulphate was used in following dilutions: 1:10E3, 1:10E4, 1 :10E5, 1:10E6 and 1:10E7. Three adult animals (3 - 5 days old) were kept in microti tre wells containing 0,75 ml of liquid medium (with E. coli and without streptom ycin sulphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with streptomycin sulphate in any concentration) ever y day (one worm in one well) beginning from third day. This investigation was ca rried out in temperature +210C and in the darkness. The obtained results are pre sented in the following table.
-
[
Worm Breeder's Gazette,
2000]
The purpose of this study was to investigate the effect of different concentrations of kanamycin sulphate in water solutions on nematode life span. In this experiment kanamycin sulphate was used in following dilutions: 1:101, 1:102, 1:103, 1:104, 1:105, 1:106 and 1:107. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and without kanamycin sulphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with kanamycin sulphate in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +210C and in the darkness.
-
[
Worm Breeder's Gazette,
2000]
The purpose of this study was to investigate the effect of different concentrations of dicaine hydrochloride in water solutions on nematode life span. In this experiment dicaine hydrochloride was used in the following dilutions: 1:104, 1:105, 1:106 and 1:107. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without dicaine hydrochloride) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with dicaine hydrochloride in any concentration) every day (one worm in one well) beginning from the third day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.
-
[
Worm Breeder's Gazette,
1995]
The purpose of the present experiment is to investigate, how low temperatures in the early stages of development can influence fecundity, length of prereproductive period, length of reproductive period, length of postreproductive period ad mean life span in the nematode C. elegans (Bristol, N2, wild type). To microtitre wells were added 0,75 ml of liquid medium with E. coli (OP 50) and 3 adult nematodes. Animals were kept during 12 hours in the room temperature, then adult nematodes were discarded and wells were stored in different temperatures during 2 days, then they were transferred in room temperature. Newly appeared worms were transferred in next wells, every well contains a single nematode. In every following day these worms were transferred in next wells. All the experiments were done in the darkness. ......................................................................... Tempe- Number Length of Mean rature of life C progeny prerepro- reproduc- postrep- span ductive tive roductive days period period period n days days days ......................................................................... 21,3 79,8+/-5,4 2,2+/-0,1 8,3+/-0,4 12,8+/-1,1 24,4+/-1,1 26 9,0 77,0+/-5,5 3,3+/-0,1 7,7+/-0,6 11,1+/-1,3 22,8+/-1,5 25 63 67,8+/-3,4 3,8+/-0,1 7,0+/-0,4 11,7+/-1,5 23,2+/-1,5 23 2,0 68,5+/-5,0 4,3+/-0,2 7,1+/-0,4 8,6+/-1,2 21,1+/-1,1 20 ..................................................................... Then, lowering of ambient temperatures during early development of nematode C. elegans resulted in decreasing of progeny. There is a decrease in the mean life span as temperature during larval development decreased, as well as the length of reproductive and the length of postreproductive periods. Then, the length of prereproductive period is increased by diminishing of the temperature of early development. These results demonstrate, that the higher is the temperature, the earlier nematodes started reproduction. These results do not support the widely accepted point of view, that lowering of temperature increases life span of different animals. There exist optimal life conditions for species, in which life span of this species is maximal. But the problem arises: what must we do in order to obtain the marked increase of longevity and, probably, immortality? Undoubtedly, it appeared reasonable to seek a new unusual experimental approaches. Acknowledment: he author wishes to express his thanks to CGC for providing C. elegans and E. coli.
-
[
Worm Breeder's Gazette,
1998]
There are data on the life span prolonging effect of pineal peptide preparation epithalamin in mice, rats and Drosophila melanogaster [1,2]. The purpose of this study was to investigate the effect of different concentrations of epithalamin in water solutions on nematode life span. In this experiment epithalamin was used in following dilutions 1:10*5, 1:10*6, 1:10*7, 1:10*8, 1:lO*9 and 1:10*10. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.75 ml of liquid medium (with E. coli and without epithalamin) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with epitalamin in any concentration) every day (one worm in one well). This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Table I Concentration of Longevity (days) epithalamin n mean +/- S.E. maximal Control 12 17.4 +/- 2.5 29 1:10*5 12 12.8 +/- 2.1 27 1:10*6 12 15.1 +/- 1.9 28 1:10*7 12 17.1 +/- 2.2 28 1:10*8 12 19.6 +/- 2.2 29 1:10*9 12 18.8 +/- 2.0 31 1:10*10 12 18.7 +/- 1.7 25
-
[
Worm Breeder's Gazette,
1996]
It is well known that the royal jelly markedly prolongs the life span of honey bees. This substance is recommended by physicians for increasing of life quality. In this experiment royal jelly extract was used in the form of "Apilac" in dilutions 0,02% and 0,002%. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without royal jelly extract) during 6 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). Beginning from third day in the whole experiment was used the medium without this royal jelly extract. A number of progeny was calculated every day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Control group Experimental Experimental group (0,02%) group (0,002%) n = 11 n = 11 n = 12 Mean +/- S.D. Mean +/- S.D. Mean +/- S.D Longevity (days): mean 19,4 +/- 1,2 18,0 +/- 1,7 21,8 +/- 1,2 maximal 25 27 26 minimal 10 9 14 Periods (days): prereproductive 2 2 2 reproductive 6,5 +/- 0,5 7,4 +/- 0,6 5,9 +/- 0,4 postreproductive 10,6 +/- 1,2 8,5 +/- 1,6 14,0 +/- 1,3 Fecundity: mean 158,8 +/- 6,6 93,9 +/- 9,9 128,9 +/- 3,4 maximal 197 179 153 minimal 115 60 111