WBPaper00034661:AgNPs_downregulated

Statistical differences between the control and exposed worms were determined by a parametric t test, and a Pearson correlation test was performed for correlation analysis, using the Statistical Package for the Social Sciences (SPSS, Chicago, IL). Genes downregulated more than 2 fold after 24 hours of AgNPs exposure.

WBPaper00034661:AgNPs_upregulated

Statistical differences between the control and exposed worms were determined by a parametric t test, and a Pearson correlation test was performed for correlation analysis, using the Statistical Package for the Social Sciences (SPSS, Chicago, IL). Genes upregulated more than 2 fold after 24 hours of AgNPs exposure.

WBPaper00031832:slr-2_regulated

P-values were calculated using either t-tests or chi-square tests, where appropriate, using the statistic language R. Pearson correlation coefficients between lin-35 and slr-2 coregulated genes as well as standard errors of mean (or deviation) for all other experiments was calculated using Microsoft Excel. A complete list of the genes that showed differential expression in a slr-2 mutant strain.

WBPaper00066447:Day8_vs_Day1_upregulated_BodyWallMuscle

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly increased expression in day 8 adults comparing to in day 1 adults in body wall muscle.

WBPaper00066447:Day8_vs_Day1_downregulated_coelomocyte

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly decreased expression in day 8 adults comparing to in day 1 adults in coelomocyte.

WBPaper00066447:Day8_vs_Day1_downregulated_intestine

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly decreased expression in day 8 adults comparing to in day 1 adults in intestine.

WBPaper00066447:Day8_vs_Day1_downregulated_neuron

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly decreased expression in day 8 adults comparing to in day 1 adults in neuron.

WBPaper00066447:Day8_vs_Day1_upregulated_coelomocyte

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly increased expression in day 8 adults comparing to in day 1 adults in coelomocyte.

WBPaper00066447:Day8_vs_Day1_downregulated_hypodermis

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly decreased expression in day 8 adults comparing to in day 1 adults in hypodermis.

WBPaper00066447:Day8_vs_Day1_upregulated_intestine

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly increased expression in day 8 adults comparing to in day 1 adults in intestine.