WBPaper00053321:PGCs_depleted_NuGen

For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. Transcripts that were depleted in embryonic primordial germ cells (PGCs) comparing to in whole embryo, according to NuGen RNAseq.

WBPaper00053321:PGCs_enriched_NuGen

For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. Transcripts that were enriched in embryonic primordial germ cells (PGCs) comparing to in whole embryo, according to NuGen RNAseq.

WBPaper00053321:nos-1(gv5)nos-2(RNAi)_upregulated_L1_SMARTseq

For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. Transcripts that showed significantly increased expression in nos-1(gv5)nos-2(RNAi) comparing to in wild type, in primordial germ cells (PGCs) at L1 larva, according to SMARTseq.

WBPaper00053321:nos-1(gv5)nos-2(RNAi)_downregulated_L1_SMARTseq

For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. Transcripts that showed significantly decreased expression in nos-1(gv5)nos-2(RNAi) comparing to in wild type, in primordial germ cells (PGCs) at L1 larva, according to SMARTseq.

WBPaper00053321:mes-2(RNAi)_upregulated_fed-L1_Truseq

For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. Transcripts that showed significantly increased expression in fed mes-2(RNAi) comparing to in starved wild type, in primordial germ cells (PGCs) at L1 larva, according to Truseq.

WBPaper00053321:embryo-to-L1_upregulated_nos-1(gv5)nos-2(RNAi)_SMARTseq

For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. Transcripts that showed significantly increased expression in L1 larva comparing to in embryo, in primordial germ cells (PGCs) of nos-1(gv5)nos-2(RNAi) animals, according to SMARTseq.

WBPaper00053321:embryo-to-L1_downregulated_nos-1(gv5)nos-2(RNAi)_SMARTseq

For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. Transcripts that showed significantly decreased expression in L1 larva comparing to in embryo, in primordial germ cells (PGCs) of nos-1(gv5)nos-2(RNAi) animals, according to SMARTseq.

WBPaper00053321:nos-1(gv5)nos-2(RNAi)_upregulated_embryo_SMARTseq

For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. Transcripts that showed significantly increased expression in nos-1(gv5)nos-2(RNAi) comparing to in wild type, in primordial germ cells (PGCs) at embryo stage, according to SMARTseq.

WBPaper00053321:nos-1(gv5)nos-2(RNAi)_downregulated_embryo_SMARTseq

For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. Transcripts that showed significantly decreased expression in nos-1(gv5)nos-2(RNAi) comparing to in wild type, in primordial germ cells (PGCs) at embryo stage, according to SMARTseq.

WBPaper00053321:mes-4(RNAi)_upregulated_fed-L1_Truseq

For differential gene expression analysis, sets of independent mutant and control mapped reads (e.g biological replicates) were used in cuffdiff analysis. The cutoff of FDR(q value)=0.05 was used as a significance cutoff for all the analyses. Transcripts that showed significantly increased expression in fed mes-4(RNAi) comparing to in starved wild type, in primordial germ cells (PGCs) at L1 larva, according to Truseq.